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US 2014/0370569 A1Published application
Expression method
AssigneeBASF SE
InventorsTobias Kueppers, Victoria Steffen, Renee Charlott Eichstadt, Stefan Evers, Karl-Heinz Maurer, Johannes Bongaerts, Hendrik Hellmuth, Thomas Weber, Timothy O'Connell
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Abstract
In a microbial fermentation, the aim is to increase the product yield of protein. This is achieved by a method in which an expression construct is introduced into a microorganism of the species Bacillus pumilus which comprises a promoter and a nucleic acid coding for the protein, and the protein is expressed in said expression construct.
Claims21
A method for producing a protein by means of a microorganism comprising
- (a) introducing an expression construct into a microorganism which comprises a promoter and a nucleic acid coding for the protein;
- (b) expression of the protein in the microorganism,
- where the microorganism belongs to the species Bacillus pumilus.
A microorganism obtainable by a method comprising
- (a) introducing an expression construct into a microorganism which comprises a promoter and a nucleic acid coding for the protein;
- (b) expression of the protein in the microorganism,
- where the microorganism belongs to the species Bacillus pumilus.
(canceled)
Dependencies are inferred from the claim text.