Modified microorganisms and methods of making butadiene using same
AssigneeBraskem S/A
InventorsMateus Schreiner Garcez Lopes, Avram Michael Slovic, Iuri Estrada Gouvea, Johana Rincones Perez, Lucas Pedersen Parizzi
Life of this patent
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Abstract
The present disclosure generally relates to microorganisms that comprise one or more polynucleotides coding for enzymes in one or more pathways that catalyze a conversion of a fermentable carbon source to butadiene. Also provided are methods of using the microorganisms in industrial processes including, for use in the production of butadiene and products derived therefrom.
Claims27
A method of producing butadiene from a fermentable carbon source, the method comprising:
- a.) providing a fermentable carbon source;
- b.) contacting the fermentable carbon source with a microorganism comprising one or more polynucleotides coding for enzymes in a pathway that catalyzes a conversion of the fermentable carbon source to one or more intermediates in a pathway for the production of butadiene, and one or more polynucleotides coding for enzymes in a pathway that catalyze a conversion of the one or more intermediates to butadiene in a fermentation media;
- and
- c.) expressing the one or more polynucleotides coding for the enzymes in the pathway that catalyzes a conversion of the fermentable carbon source to one or more intermediates in a pathway for the production of butadiene and the one or more polynucleotides coding for enzymes in a pathway that catalyze a conversion of the one or more intermediates to butadiene in the microorganism to produce butadiene,
- wherein the one or more intermediates in the pathway for the production of butadiene include at least one of propionyl-CoA and formic acid.
(canceled)
A microorganism comprising one or more polynucleotides coding for enzymes in a pathway that catalyzes a conversion of a fermentable carbon source to one or more intermediates in a pathway for the production of butadiene and one or more polynucleotides coding for enzymes in a pathway that catalyze a conversion of the one or more intermediates to butadiene, wherein the one or more intermediates in the pathway for the production of butadiene include at least one of propionyl-CoA and formic acid.
(canceled)
Dependencies are inferred from the claim text.