Compositions and methods for the relief of inhibition of aldehyde decarbonylase
AssigneeBrookhaven Science Associates, LLC
InventorsJohn Shanklin, Carl Andre
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Abstract
The present invention is related to compositions and methods for enhanced synthesis of hydrocarbons, particularly, but not limited to, alkanes. The invention, in one embodiment, utilizes the co-expression of a hydrogen peroxide metabolizing enzyme in the presence of an aldehyde decarbonylase enzyme to relieve hydrogen peroxide inhibition of the aldehyde decarbonylase enzyme by hydrogen peroxide. In a preferred embodiment a catalase-aldehyde decarbonylase expression construct and fusion peptide is used. The present invention also relates to microorganisms engineered to express said enzymes and to produce hydrocarbon molecules.
Claims28
A method for enhancing the production of one or more of alkanes and alkenes in a bioengineered microorganism, said method comprising:
- i) transforming a microorganism to express an aldehyde decarbonylase enzyme and a peroxide metabolizing enzyme and, ii) culturing said transformed microorganism under conditions and for a length of time suitable for the production of one or more of alkanes and alkenes.
A composition comprising an engineered hybrid protein, said protein comprising an aldehyde decarbonylase enzyme and a peroxide metabolizing enzyme.
A composition comprising an expression construct encoding an aldehyde decarbonylase enzyme and a peroxide metabolizing enzyme.
A composition comprising a microorganism engineered to express an aldehyde decarbonylase enzyme and a peroxide metabolizing enzyme.
A cell-free production system for producing alkanes and alkenes, said cell-free system comprising an aldehyde decarbonylase enzyme, a peroxide metabolizing enzyme and an aldehyde substrate.
A method for relieving hydrogen peroxide inhibition of aldehyde decarbonylase, said method comprising providing a peroxide metabolizing enzyme in a reaction mixture wherein said reaction mixture comprises an aldehyde decarbonylase enzyme and an aldehyde substrate.
Dependencies are inferred from the claim text.