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US 2014/0370562 A1Published application
Production of beta-phellandrene using genetically engineered cyanobacteria
AssigneeThe Regents of the University of California
InventorsAnastasios Melis, Fiona K. Bentley, Hsu-Ching Chen Wintz, Andreas Zurbriggen
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Abstract
The present invention provides methods and compositions for producing β-phellandrene hydrocarbons from cyanobacteria.
Claims24
A method of obtaining β-phellandrene from cyanobacteria, the method comprising:
- culturing a cyanobacteria strain that has been genetically modified to express a heterologous β-phellandrene synthase under conditions in which the β-phellandrene synthase is expressed;
- and
- isolating β-phellandrene hydrocarbons produced in the cyanobacteria that has diffused from the cyanobacteria into the culture medium.
An isolated nucleic acid encoding β-phellandrene synthase, wherein the nucleic acid has at least 95% identity to SEQ ID NO:3.
A cell culture comprising a cyanobacteria strain, wherein the cyanobacteria strain is genetically modified to express a heterologous β-phellandrene synthase;
- and cell culture media comprising β-phellandrene produced in the cyanobacteria that has diffused into the cell culture media from the cyanobacteria.
A method of obtaining β-phellandrene, the method comprising:
- introducing an expression construct comprising a nucleic acid sequence encoding β-phellandrene synthase into a cyanobacteria host cell;
- culturing the cyanobacterial host cell under conditions in which the β-phellandrene synthase is expressed;
- and
- isolating β-phellandrene produced by the cyanobacterial host cell that has diffused from the cyanobacterial host cell into the culture media.
1 more element
Dependencies are inferred from the claim text.