Modifying soybean oil composition through targeted knockout of the fad2-1a/1b genes
AssigneeCellectis
InventorsLuc Mathis, Daniel F. Voytas, Feng Zhang, William Haun
Life of this patent
Estimating the term…
Abstract
Materials and methods are provided for making soybean varieties that have altered oil composition as a result of mutations in the FAD2-1A and FAD2-1B genes.
Claims29
A soybean plant, plant part, or plant cell comprising a mutation in one or more FAD2-1A alleles, a mutation in one or more FAD2-1B alleles, or a mutation in one or more FAD2-1A alleles and a mutation in one or more FAD2-1B alleles, wherein said plant, plant part, or plant cell produces oil that has increased oleic acid content and decreased linoleic acid content as compared to oil produced from a corresponding wild type soybean plant, plant part, or plant cell.
5-6. (canceled)
(canceled)
A soybean plant, plant part, or plant cell comprising a mutation in one or more FAD2-1A alleles, a mutation in one or more FAD2-1B alleles, or a mutation in one or more FAD2-1A alleles and a mutation in one or more FAD2-1B alleles, wherein said plant, plant part, or plant cell produces oil that has an oleic acid content of greater than 30%.
11-12. (canceled)
A soybean plant, plant part, or plant cell comprising a mutation in one or more FAD2-1A alleles, a mutation in one or more FAD2-1B alleles, or a mutation in one or more FAD2-1A alleles and a mutation in one or more FAD2-1B alleles, wherein said plant, plant part, or plant cell produces oil that has a linoleic acid content of less than 10%.
15-16. (canceled)
A method for producing soybean oil having increased oleic acid content and reduced linoleic acid content, comprising:
- (a) providing a soybean plant or plant part comprising a mutation in one or more FAD2-1A alleles, a mutation in one or more FAD2-1B alleles, or a mutation in one or more FAD2-1A alleles and a mutation in one or more FAD2-1B alleles;
- and
- (b) producing oil from said plant or plant part.
(canceled)
A method for making a soybean plant comprising a mutation in each FAD2-1A allele and a mutation in each FAD2-1B allele, said method comprising:
- (a) contacting a population of soybean plant cells comprising functional FAD2-1A and FAD2-1B alleles with one or more rare-cutting endonucleases targeted to endogenous FAD2-1A sequences, and one or more rare-cutting endonucleases targeted to endogenous FAD2-1B sequences,
- (b) selecting, from said population, a cell in which each FAD2-1A allele and each FAD2-1B allele has been inactivated, and
- (c) regenerating said selected plant cell into a soybean plant.
(canceled)
(canceled)
A method for generating a soybean plant comprising a mutation in each FAD2-1A allele and a mutation in each FAD2-1B allele, comprising:
- (a) crossing a first soybean plant comprising a mutation in at least one FAD2-1A allele and a mutation in at least one FAD2-1B allele with a second soybean plant comprising a mutation in at least one FAD2-1A allele and a mutation in at least one FAD2-1B allele, to obtain progeny;
- and
- (b) selecting from said progeny a soybean plant that comprises a mutation in each FAD2-1A and FAD2-1B allele.
32-33. (canceled)
Dependencies are inferred from the claim text.