Method for producing an examination reagent and kit for analysing a t-cell frequency
AssigneeJacobs University Bremen GmbH
InventorsSebastien Hartmut Springer, Sunil Kumar Saini
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Abstract
A method for producing an examination reagent includes adding a helper ligand to an unfolded receptor protein in an initial solution so as to provide a pre-solution of the examination reagent with a folded receptor protein. The folded receptor protein comprises a bonded helper ligand which can be exchanged with an examination peptide.
Claims23
1-32. (canceled)
A method for producing an examination reagent, the method comprising:
- adding a helper ligand to a receptor protein which is unfolded in an initial solution so as to provide a pre-solution of the examination reagent with a receptor protein which is folded,
- wherein, the receptor protein which is folded comprises a bonded helper ligand which can be exchanged with an examination peptide.
A receptor protein which is folded comprising at least one of a helper ligand bonded to the receptor protein and a ligand exchanger/ligand releaser which has an affinity for the receptor protein which is folded which is less than an affinity for the helper ligand,
- wherein, the helper ligand is a compound forming a receptor protein which is exchangeable and folded, and
- the helper ligand is a peptide comprising 2, 3, 4, 5, 6, 7, 8, 9, 10 or 11 amino acids.
A pre-solution comprising:
- a receptor protein which is folded comprising at least one of a helper ligand bonded to the receptor protein and a ligand exchanger/ligand releaser which has an affinity for the receptor protein which is folded which is less than an affinity for the helper ligand, wherein, the helper ligand is a compound forming a receptor protein which is exchangeable and folded, and the helper ligand is a peptide comprising 2, 3, 4, 5, 6, 7, 8, 9, 10 or 11 amino acids, or
- a multimer comprising a plurality of receptor proteins each of which is folded, wherein the plurality of receptor proteins each of which is folded comprises at least one of a helper ligand bonded to the receptor protein and a ligand exchanger/ligand releaser which has an affinity for the receptor protein which is folded which is less than an affinity for the helper ligand, wherein, the helper ligand is a compound forming a receptor protein which is exchangeable and folded, and the helper ligand is a peptide comprising 2, 3, 4, 5, 6, 7, 8, 9, 10 or 11 amino acids, or
- a particle comprising a receptor protein which is folded comprising at least one of a helper ligand bonded to the receptor protein and a ligand exchanger/ligand releaser which has an affinity for the receptor protein which is folded which is less than an affinity for the helper ligand, wherein, the helper ligand is a compound forming a receptor protein which is exchangeable and folded, and the helper ligand is a peptide comprising 2, 3, 4, 5, 6, 7, 8, 9, 10 or 11 amino acids, or
- a membrane preparation comprising a receptor protein which is folded comprising at least one of a helper ligand bonded to the receptor protein and a ligand exchanger/ligand releaser which has an affinity for the receptor protein which is folded which is less than an affinity for the helper ligand, wherein, the helper ligand is a compound forming a receptor protein which is exchangeable and folded, and the helper ligand is a peptide comprising 2, 3, 4, 5, 6, 7, 8, 9, 10 or 11 amino acids.
Dependencies are inferred from the claim text.