A method of preparing biological material
AssigneeNorth-West University
InventorsAnne Frederica Grobler, Oksana Levanets
Life of this patent
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Abstract
This invention pertains to a method of preparing biological material from a biological sample selected from the group consisting of blood and sputum samples. The method includes the step of altering at least one constitutive characteristic of the biological sample in the presence of a capturing scaffold by adding a lysis buffer containing a solubilising agent and a detergent to the biological sample, for simultaneously inhibiting coagulation of the biological sample; lysing the biological sample to release the biological material from the biological sample, thus making the biological material available; and capturing at least one fraction of the biological material on the capturing scaffold.
Claims26
A method of preparing biological material from a biological sample selected from the group consisting of blood and sputum samples, the method including the step of altering at least one constitutive characteristic of the biological sample in the presence of a capturing scaffold by adding a lysis buffer containing a solubilising agent and a detergent to the biological sample, for simultaneously inhibiting coagulation of the biological sample;
- lysing the biological sample to release biological material from the biological sample thus making the biological material available;
- and capturing at least one fraction of the biological material on the capturing scaffold.
A method of preparing biological material from a biological sample substantially as herein described and exemplified with reference to the accompanying drawing.
A capturing scaffold for capturing a biological sample substantially as herein described and exemplified and as illustrated in the accompanying drawing.
A kit for use in a method of preparing biological material from a biological sample substantially as herein described and exemplified and as illustrated in the accompanying drawing.
Dependencies are inferred from the claim text.