Saliva-derived measures of telomere abundance and sample collection device
AssigneeTelome Health, Inc.
InventorCalvin Harley
Life of this patent
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Abstract
This invention provides devices and methods for sample collection. Devices can include (a) a container having an opening and adapted to receive a liquid sample through the opening; (b) a cover configured to reversibly seal the opening; and (c) a capture device configured to be introduced into the container, wherein the capture device is configured to selectively bind cells of a first type and not to substantially bind cells of a second cell type. The sample can be analyzed to make a measure of telomere abundance and the abundance can be correlated to a measure of health.
Claims69
A kit comprising:
- (a) a container having an opening adapted to receive a liquid sample through the opening;
- (b) a cover configured to reversibly seal the opening;
- and
- (c) a capture device configured to be introduced into the container, wherein the capture device is configured to selectively bind cells of a first type and not to substantially bind cells of a second cell type.
A method comprising:
- introducing saliva into a container;
- and
- removing epithelial cells from the saliva in the container, wherein at least one cell type selected from myeloid cells and lymphoid cells is retained in the saliva in the container.
A method comprising:
- introducing saliva into a container;
- and
- capturing epithelial cells in the saliva in the container with capture particles having moieties that bind epithelial cells, wherein at least one cell type selected from myeloid cells and lymphoid cells remains retains free in the saliva in the container.
A method comprising determining a measure of telomere abundance on cells from a saliva sample, wherein the cells are substantially free of epithelial cells.
A kit comprising:
- a container having an opening and adapted to receive a liquid sample;
- a cover configured to reversibly seal the opening;
- and
- capture particles having binding moieties that bind epithelial cells but that do not substantially bind at least one of myeloid cells and lymphoid cells.
A method comprising:
- determining a measure of telomere abundance in saliva-derived cells from a subject sample;
- and
- correlating the measure with:
- (1) a measure of health;
- (2) a risk of a pathological condition;
- (3) a telomeric disease or (4) drug responsiveness.
The method of claim 378 comprising correlating the measure with a telomeric disease.
A method for monitoring the status of a subject comprising:
- determining measures of telomere abundance from cells in each of saliva-derived subject sample in a plurality of saliva-derived subject samples taken over a period of time;
- determining differences in the measures;
- and
- correlating the differences with progression of a telomeric disease, wherein decreases in the measures indicates progression of the disease.
A method comprising:
- sending a container comprising a subject sample comprising saliva;
- and
- receiving an electronically transmitted signal containing information indicating a determination of an measure of telomere abundance in the sample or a correlation of an measure of telomere abundance with:
- (1) a measure of health;
- (2) a risk of a pathological condition;
- (3) a telomeric disease or (4) drug responsiveness.
A method comprising:
- determining a rate of change in a measure of telomere abundance in saliva-derived cells from a plurality of subject samples, each sample taken at different times;
- and
- correlating the rate of change with:
- (1) a measure of health;
- (2) a risk of a pathological condition;
- (3) a telomeric disease or (4) drug responsiveness.
Dependencies are inferred from the claim text.