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US 2014/0370501 A1Published application
Hydrolase enzyme substrates and uses thereof
AssigneeGeneral Atomics
InventorsChong-Sheng YUAN, Xiaoru CHEN
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Abstract
The present invention provides novel methods for determining the presence or amount of a hydrolytic enzyme in a sample, based on novel substrates for the enzymes, and also provides compositions and methods that provide highly sensitive assay methods for such hydrolytic enzymes.
Claims22
A hydrolytic enzyme substrate, which is a compound of formula (I):
- wherein:
- A is an aromatic or heteroaromatic group, a 1-alkene or a 1-alkyne, each of which is optionally substituted;
- each R is independently H or an optionally substituted C1-C4 alkyl or aryl;
- n is an integer from 1-4;
- and X is a group comprising a substrate moiety,
- wherein the substrate moiety comprises a recognition component of a substrate for the hydrolytic enzyme, and wherein the activity of said hydrolytic enzyme is capable of hydrolyzing said compound of formula (I) to form compounds (II) and (III):
3 more elements
2-10. (canceled)
12-16. (canceled)
Dependencies are inferred from the claim text.