Recombinant mycobacteriophages for delivery of nucleic acids of interest into mycobacteria
AssigneesAlbert Einstein College of Medicine of Yeshiva University, The University of Pittsburgh of the Commonwealth System of High Education
InventorsWilliam R. Jacobs, Graham F. Hatfull
Life of this patent
Estimating the term…
Abstract
Methods are provided for detecting mycobacteria in a sample, including clinical samples. Methods are also provided for determining susceptibility of mycobacterial strains to known or potential antibiotic agents, as are kits therefor. Recombinant mycobacteriophages are also provided comprising heterologous nucleic acids of interest.
Claims26
An isolated recombinant mycobacteriophage comprising a vector backbone into which (a) heterologous mycobacteriophage promoter nucleic acid and (b) a heterologous nucleic acid encoding a protein of interest are integrated, and wherein (a) is upstream of (b), and wherein the vector backbone is derived from a mycobacteriophage TM4 and the recombinant mycobacteriophage is conditionally propagating.
An isolated recombinant mycobacteriophage comprising a vector into which (a) heterologous PLeft lytic promoter of mycobacteriophage L5 and (b) a heterologous nucleic acid encoding a reporter protein are integrated, and wherein (a) is upstream of (b).
7-18. (canceled)
20-26. (canceled)
(canceled)
(canceled)
39-47. (canceled)
A method for producing recombinant mycobacteriophages comprising:
- a) introducing a vector into a mycobacterium so as to form a lysogen, which vector comprises (i) a mycobacteriophage promoter nucleic acid and (ii) a heterologous nucleic acid encoding a protein of interest, and wherein (i) is upstream of (ii), and integrated into a vector backbone derived from a mycobacteriophage TM4, and is temperature-sensitive conditionally propagating;
- b) maintaining the lysogen at a temperature that permits the lysogen to replicate into a plurality of lysogens;
- and then
- c) maintaining the plurality of lysogens at a temperature that effects induction in the plurality of lysogens, so as thereby to produce recombinant mycobacteriophages comprising (i) and (ii).
51-65. (canceled)
Dependencies are inferred from the claim text.