Bile resistant bacillus composition
AssigneeChr. Hansen A/S
InventorsInge KNAP, Ane KNARREBORG, Thomas Dyrmann LESER, Lund BENTE, Mette Dines CANTOR, Patrick DERKX
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Abstract
A bacillus composition characterized by fast germination and outgrowth in bile salts (simulated gut environment) and by producing a compound of interest. The bacillus composition may be used as supplement in animal feed where it has a probiotic (health promoting) effect and increases the digestion and availability of nutrients from animal feeds.
Claims11
1-12. (canceled)
A Bacillus composition comprising from 105 to 1012 CFU/g Bacillus spore cells of a mutant Bacillus strain, wherein:
- (a) the Bacillus spores have a germination and outgrowth from spore to vegetative cell in the presence of a bile salt medium comprising 4 mM or 6 mM bile salt such that the spores reach a vegetative cell growth point of 0.4 OD630 within less than 18 hours or 19 hours, respectively;
- and
- (b) the Bacillus vegetative cells overproduce at least one desired compound in comparison to the wild-type Bacillus strain from which the mutant Bacillus strain derives when grown under comparable conditions.
A method for isolating a mutant Bacillus strain which germinates and outgrows rapidly in the presence of a bile salt and which overproduces at least one desired compound, comprising:
- (a) subjecting a wild-type Bacillus spore cell to mutagenesis to isolate a Bacillus spore cell having a germination and outgrowth from spore to vegetative cell in the presence of a bile salt medium comprising 4 mM or 6 mM bile salt such that the Bacillus spores reach a vegetative cell growth point of 0.4 OD630 within less than 18 hours or 19 hours;
- (b) subjecting vegetative cells grown from the isolated spore cell of step (a) to mutagenesis, thereby obtaining a pool of individual vegetative cells which retain rapid germination and outgrowth in the presence of a bile salt;
- and
- (c) selecting a vegetative cell from the pool of step (b), which vegetative cell retains rapid germination and outgrowth in the presence of a bile salt, and which overproduces the at least one desired compound.
A method for feeding an animal comprising administering the Bacillus composition of claim 1 to the animal.
Dependencies are inferred from the claim text.