Arsenic Compound Solution and Albumin Nanoparticle and Lyophilized Preparation Entrapping Arsenic Compound Prepared Using Same

A method for preparing arsenic compound-loaded albumin nanoparticles, wherein said method comprises:

• (a) preparing an albumin solution with deionized water and albumin which is human albumin or bovine albumin, and making the concentration of the albumin solution be from 0.01 to 0.02 g/mL;
• (b) mixing the arsenic compound solution and the albumin solution with a mass ratio of As2O3 added to prepare the arsenic compound solution to the albumin solution ranging from 1:5 to 1:20, adjusting the pH value of the mixture to from 7.5 to 9 with a NaOH solution;
• and adding a dehydration agent, which is selected from the group consisting of ethanol, acetone, and any combination thereof, to the mixture with a volume ratio of the dehydrating agent to the albumin solution ranging from 2.0:1 to 3.5:1 while stirring, to obtain an emulsion;
• (c) placing the emulsion in a water bath, and subjecting the emulsion to physical curing at a curing temperature between 40° C. and 90° C. for 10 min to 240 min, in order to obtain an emulsion of nanoparticles;
• and
• (d) subjecting the emulsion of nanoparticles to evaporation to remove the dehydrating agent, and then subjecting the resulting emulsion to centrifugal separation to obtain arsenic compound-loaded albumin nanoparticles.