Artificial antigen presenting cells having a defined and dynamic shape
AssigneeThe Johns Hopkins University
InventorsJordan J. Green, Joel C. Sunshine, Karlo Perica, Jonathan Schneck
Life of this patent
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Abstract
Compositions and methods comprising asymmetrical artificial antigen presenting cells (aAPCs) are disclosed. The non-spherical aAPCs more closely mimic endogenous cell-cell interactions and can be used for antigen-specific immunotherapy.
Claims47
An artificial antigen presenting cell (aAPC) comprising:
- (a) a three-dimensional microparticle or nanoparticle having an asymmetrical shape that mimics a shape of a cell or a microorganism;
- and
- (b) one or more molecules capable of interacting with one or more T cell receptors (TCRs) of a T cell.
A method for modulating a T cell, the method comprising:
- (a) providing an artificial antigen presenting cell (aAPC) comprising:
- (i) a three-dimensional microparticle or nanoparticle having an asymmetrical shape defined by a dimension (a) along an x-axis, a dimension (b) along a y-axis, and a dimension (c) along a z-axis, wherein at least one of (a), (b), or (c) is not equal to at least one other dimension (a), (b), or (c);
- and
- (ii) one or more molecules capable of interacting with one or more T cell receptors (TCRs) of a T cell and one or more molecules capable of interacting with a receptor other than a TCR on the T cell to costimulate the T cell;
- (b) contacting the T cell with the aAPC;
- and
- (c) allowing the T cell to be modulated.
A method for making an artificial antigen presenting cell (aAPC) comprising a three-dimensional microparticle or nanoparticle having an asymmetrical shape defined by a dimension (a) along an x-axis, a dimension (b) along a y-axis, and a dimension (c) along a z-axis, wherein at least one of (a), (b), or (c) is not equal to at least one other dimension (a), (b), or (c);
- and one or more molecules capable of interacting with one or more T cell receptors (TCRs) of a T cell, the method comprising:
- (a) providing or preparing a plurality of microparticles or nanoparticles;
- (b) preparing a film comprising the plurality of microparticles or nanoparticles;
- (c) stretching the film comprising the plurality of microparticles or nanoparticles to form a plurality of three-dimensional microparticles or nanoparticles having an asymmetrical shape;
- (d) harvesting the plurality of three-dimensional microparticles or nanoparticles having an asymmetrical shape;
- and
- (e) coupling to the plurality of three-dimensional microparticles or nanoparticles having an asymmetrical shape one or more molecules capable of interacting with one or more T cell receptors (TCRs) of a T cell.
A device for stretching a film, the device comprising:
- at least a first and a second block adapted to immobilize at least two edges of a film, the at least first and second blocks comprising one or more surfaces in contact with the film;
- the at least first and second blocks positioned parallel to one another and perpendicular and in operational communication with at least two parallel rods, wherein at least the second block is adapted to be selectively positioned along the at least two parallel rods relative to the first block;
- the device further comprising one or more of (i) a cord attached to the second block and (ii) a screw attached to the cord, each of which is adapted to selectively position the second block along the at least two parallel rods relative to the first block.
Dependencies are inferred from the claim text.