Back to results
US 2014/0370070 A1Published application
Methods for Producing Hair Microfollicles and De Novo Papillae and Their Use for In Vitro Tests and In Vivo Implantations
AssigneeTechnische UniversitäT Berlin
InventorsGerd LINDNER, Roland LAUSTER
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method for producing hair microfollicles comprising the steps of: a) providing de novo papillae, b) providing other cell populations selected from the group of fibroblasts, keratinocytes and melanocytes, and co-culturing the de novo papillae with at least one other cell population in non-adherent culture vessels. The present invention relates also to methods of producing de novo papillae usable in said method for producing hair microfollicles.
Claims8
De novo papillae produced by a method comprising the steps of:
- (a) providing at least one dermal papilla (DP) from at least one mammal hair follicle;
- (b) isolating dermal hair papilla fibroblasts (DPFs) from the DP by mechanically fixing said DP at the surface of a cell culture vessel, whereby the basal lamina is perforated to allow said DPFs migrating out;
- (c) expanding the isolated DPFs in monolayer culture without collagen coating, wherein said DPFs are passaged at least once;
- (d) condensing the expanded DPFs in non-adhesive culture vessels in a cell concentration per vessel surface of 1,000 to 100,000 DPFs/cm2 into cell aggregates and differentiating the cell aggregates into cell aggregates that exhibit the size and shape of the physiological DP in a non-adhesive culture vessel;
- and
- (e) coating the cell aggregates produced in (d) with extracellular matrix proteins, thereby producing de novo papillae.
3 more elements
Dependencies are inferred from the claim text.