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US 2014/0370042 A1Published application
Stabilized Antiviral Fusion Helices
AssigneeDana-Farber Cancer Institute, Inc.
InventorsLoren D. Walensky, Gregory H. Bird
Life of this patent
Estimating the term…
Abstract
Internally cross-linked peptides useful for interfering with Respiratory Syncytial Virus (RSV) infection is based on RSV-F protein. These peptides are capable of reducing infection in cellular and animal models.
Claims38
An internally cross-linked polypeptide comprising the amino acid sequence A0B0C0D0E0F0G0 A1B1C1D1E1F1G1 A2B2C2D2E2F2G2 A3B3C3D3E3F3G3 A4B4C4D4E4F4G4 (SEQ ID NO:1) wherein:
- A0 is Phe, or a conservative amino acid substitution;
- B0 is Asp, or any amino acid (e.g., any of the 20 naturally occurring amino acids);
- C0 is Ala, or Ser, or any amino acid;
- D0 is Ser, or a conservative amino acid substitution;
- E0 is Ile, or a conservative amino acid substitution;
- F0 is Ser, or any amino acid;
- G0 is Gln, or any amino acid;
- A1 is Val or Ile, or a conservative amino acid substitution;
- B1 is Asn, or any amino acid;
- C1 is Glu, or any amino acid;
- D1 is Lys, or a conservative amino acid substitution;
- E1 is Ile, or a conservative amino acid substitution;
- F1 is Asn, or any amino acid;
- G1 is Gln, or any amino acid;
- A2 is Ser, or a conservative amino acid substitution;
- B2 is Leu, or a conservative amino acid substitution;
- C2 is Ala, or any amino acid;
- D2 is Phe, or a conservative amino acid substitution;
- E2 is Ile, or a conservative amino acid substitution;
- F2 is Arg, or any amino acid;
- G2 is Lys, or Arg, or any amino acid;
- A3 is Ser, or a conservative amino acid substitution;
- B3 is Asp or Asn, or a conservative amino acid substitution;
- C3 is Glu, or any amino acid;
- D3 is Leu, or a conservative amino acid substitution;
- E3 is Leu, or a conservative amino acid substitution;
- F3 is His, or any amino acid;
- G3 is Asn or His, or any amino acid;
- A4 is Val or Ile, or a conservative amino acid substitution;
- B4 is Asn, or a conservative amino acid substitution;
- C4 is Ala, Val, or Thr, or any amino acid;
- D4 is Gly, or a conservative amino acid substitution;
- E4 is Lys, or a conservative amino acid substitution;
- F4 is Ser, or any amino acid;
- and G4 is Thr, or any amino acid;
- wherein
- the side chains of two amino acids separated by two or six amino acids are replaced by an internal staple;
- the side chains of three amino acids are replaced by internal staples and/or an internal stitch;
- the side chains of four amino acids are replaced by internal staples, internal stitches, or a combination of internal staples and stitches;
- or
- the side chains of at least four amino acids are replaced by internal staples, internal stitches, or a combination of internal staples and stitches.
38 more elements
The method of claim 35, comprising;
- assessing a level of RSV or a symptom associated with RSV in the subject before and after treatment;
- and
- continuing treatment until a decrease in the level of RSV after treatment.
Dependencies are inferred from the claim text.