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AssigneeUniversitäT ZüRich
InventorsLars Hangartner, Arkadiusz Wyrzucki
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The invention relates to a method for isolating a polypeptide reactive to influenza A hemagglutinin, comprising the steps of preparing a library of nucleic acid sequences, wherein each member of said library is attached to a polypeptide sequence encoded thereby, selecting members of said library by contacting the members with a panning antigen, wherein the panning antigen comprises a hemagglutinin stem region polypeptide sequence, subsequently removing members unreactive to the panning antigen, and selecting a member of said library by determining its binding to at least 5 influenza A hemagglutinin subtypes of subtype group 1 and 2. The invention further relates to polypeptides, particularly antibodies, obtainable by this method, that neutralize at least five influenza A subtypes of subtype group 1 and 2, to vaccines and uses thereof.
A method for isolating a polypeptide reactive to influenza A hemagglutinin, comprising the steps of
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A vaccine comprising a hemagglutinin polypeptide having a stem sequence and an apical sequence, characterized in that said stem sequence is exposed and said apical sequence is attached at residue 150 to 170 to a globular structure or to a surface structure by a tether having less than 4 nm in length.
A vaccine comprising an antigen comprising a first and a second hemagglutinin polypeptide, each hemagglutinin polypeptide having a stem sequence and an apical sequence, wherein each of said stem sequences is exposed and said apical sequences are attached to one another at residue 150 to 170 by a tether having less than 4 nm in length.
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Dependencies are inferred from the claim text.