Biomarkers for Tuberculosis and HIV/AIDS
OwnerAndries J.C. Steyn, Michael Kimerling +2inventors
InventorsAndries J.C. Steyn, Michael Kimerling, German Henostroza, David K. Crossman
Life of this patent
Estimating the term…
Abstract
Described herein is a diagnostic test that identifies circulating biomarkers for the differentiation and classification of the pathogenesis of TB and/or HIV in a population.
Claims10
A method of diagnosing a subject as HIV+ or HIV− comprising:
- a) measuring the levels of eotaxin, SCF, PDFGbb in a sample from the subject, b) computing a predictive value utilizing the following equation:
- p
- =
- 1
- 1
- +
-
- -
- z
- where z=5.654+0.003*Eotaxin−0.032*SCF−0.001*PDGF ββ
- wherein a value >0.5 predicts HIV+, and a value <0.5 predicts HIV−, thus diagnosing the subject as HIV+ or HIV−.
A method of diagnosing a subject as TB+ or TB− comprising a) measuring the levels of MCSF, TNFBeta, MCP3, GROalpha in a sample from a subject, b) computing a predictive value utilizing the following equation:
- p
- =
- 1
- 1
- +
-
- -
- z
- where z=2.146+0.066*MCSF+0.593*TNFβ−0.058*MCP3+0.012GROα
- wherein a value >0.5 predicts TB+, and a value <0.5 predicts TB−, thus diagnosing the subject as TB+ or TB−.
A method of diagnosing a subject as PPD+ or not PPD+ comprising a) measuring the levels of LIF, MCP3, CTACK and ICAM-1 in a sample from a subject, b) computing a predictive value utilizing the following equation:
- p
- =
- 1
- 1
- +
-
- -
- z
- where z=−0.611−0.055*LIF+0.009*MCP3+0.001*CTACK−0.141*ICAM-1/1000
- wherein a value >0.5 predicts PPD+, and a value <0.5 predicts not PPD+, thus diagnosing the subject as PPD+ or not PPD+.
A method of diagnosing the HIV status and the TB status in a subject comprising:
- a) measuring the levels of eotaxin, SCF, PDFGbb in a sample from the subject;
- and
- b) computing a predictive value utilizing the following equation:
- p
- =
- 1
- 1
- +
-
- -
- z
- where z=5.654+0.003*Eotaxin−0.032*SCF−0.001*PDGF ββ
- wherein a value >0.5 predicts HIV+, and a value <0.5 predicts HIV−, thus diagnosing the subject as HIV+ or HIV−;
- c) measuring the levels of MCSF, TNFBeta, MCP3, GROalpha in a sample from the subject;
- and
- d) computing a predictive value utilizing the following equation:
- p
- =
- 1
- 1
- +
-
- -
- z
- where z=2.146+0.066*MCSF+0.593*TNFβ−0.058*MCP3+0.012GROα
- wherein a value >0.5 predicts TB+, and a value <0.5 predicts TB−, thus diagnosing the subject as TB+ or TB−.
Dependencies are inferred from the claim text.