Removal of tumor cells from intraoperative autologous blood salvage
AssigneeTrion Pharma GmbH
InventorsHorst Lindhofer, Michael Stroehlein, Markus Heiss
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Abstract
The invention relates to a method performed ex vivo for removal of tumor cells from intraoperatively collected blood salvage, to antibodies and scaffold proteins which mimic antibodies for use in said ex vivo method, to the use of said ex vivo method for removal of tumor cells from intraoperatively collected blood salvage followed by reintroducing the so obtained purified blood salvage or of concentrates of erythrocytes purified by said method to a patient from whom said intra-operatively collected blood was obtained, as well as to blood salvage or a concentrate of erythrocytes, both obtainable by said method for reinfusion to said patient.
Claims17
Ex vivo-method for removal of tumor cells from intra-operatively salvaged blood comprising the following steps:
- providing intra-operatively salvaged blood;
- contacting said intra-operatively salvaged blood with
- at least one antibody selected from the group consisting of a bispecific, trispecific, tetraspecific and multispecific antibody, and a bivalent, trivalent, tetravalent and multivalent antibody, and/or
- with at least a scaffold protein with multispecific or multivalent binding properties similar to antibodies,
- wherein the at least one antibody and/or the at least one scaffold protein is contacted with said intra-operatively salvaged blood for a time period which is sufficient to cross-link tumor cells and optionally immune cells and/or other tumor cells in order to obtain associates, wherein said tumor cells, said other tumor cells and said immune cells are potentially present in the intra-operatively salvaged blood, and wherein said tumor cells are specifically recognized by said at least one antibody or said at least one scaffold protein;
- and
- removing said associates from said intra-operatively salvaged blood.
Dependencies are inferred from the claim text.