Methods for autologous stem cell transplantation
AssigneeMayo Foundation for Medical Education and Research
InventorsSvetomir N. Markovic, Luis F. Porrata
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Abstract
Materials and methods for obtaining populations of lymphocytes and administering the population of lymphocytes to a subject are disclosed herein. In particular, disclosed herein are materials and methods for obtaining lymphocyte populations that contain at least about 0.5×109 NK cells per kilogram weight of the subject from which the cells are harvested.
Claims43
A method for treating a patient, said method comprising:
- a) collecting from said patient a biological sample comprising NK cells;
- b) monitoring the number of collected NK cells;
- c) repeating steps (a) and (b) until the total number of collected NK cells is at least 0.5×109 cells per kg;
- and
- d) returning said collected NK cells to said patient.
A method for treating a patient, said method comprising:
- a) administering autologous lymphocytes to said patient, wherein said autologous lymphocytes are administered in an amount of at least 0.5×109 cells/kg;
- b) monitoring the number of NK cells within said patient;
- and
- c) if said number of NK cells at day 15 is less than 80 cells/μL of blood, administering to said patient one or more agents to stimulate NK cell function or activity.
A method for obtaining a population of lymphocytes, said method comprising:
- a) collecting from a subject a biological sample comprising lymphocytes;
- b) monitoring the number of NK cells within the collected lymphocytes;
- and
- c) repeating steps (a) and (b) until the total number of NK cells collected from said subject is at least 0.5×109 cells/kg.
A container comprising a population of lymphocytes removed from a subject, wherein said population comprises an amount of NK cells that is at least 0.5×109 cells/kg, and wherein said container comprises an identifier corresponding to said subject.
A container comprising an inner surface, wherein one or more agents are dispersed on said inner surface, and wherein said one or more agents stimulate NK cell function or activity.
Dependencies are inferred from the claim text.