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US 2014/0369937 A1Published application
Activatable nanoprobes for intracellular drug delivery
AssigneeUniversity of Central Florida Research Foundation, Inc.
InventorsSwadeshmukul Santra, James Turkson
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Abstract
An activatable nanoprobe is provided having a core component and an active agent associated with the core component via a bond configured to be cleaved upon exposure to an endogenous compound.
Claims19
A method for monitoring intracellular drug delivery within a subject comprising:
- administering to the subject an effective amount of an optically activatable nanoprobe, the optically activatable nanoprobe comprising:
- a core component comprising a quantum dot;
- and
- an active agent linked to the quantum dot;
- wherein, upon intracellular uptake of the nanoprobe, a linkage between the active agent and the core component is cleaved to allow for release of the active agent and to allow the quantum dot to transfer from a quenched state, wherein the luminescence of the plurality of quantum dot is quenched, to a luminescent state, wherein the luminescence of the plurality of quantum dot is activated;
- and
- detecting a presence of the quantum dot, wherein an increase in luminescence of the quantum dot is indicative of a release of the active agent intracellularly.
4 more elements
A method for monitoring intracellular drug delivery within a subject in whom an effective amount of an optically activatable nanoprobe has been administered, the optically activatable nanoprobe comprising:
- an inorganic core;
- a plurality of quantum dots linked to the inorganic core, and
- at least one ligand linked to respective ones of the plurality of quantum dots, the at least one ligand comprising at least an active agent linked to the quantum dot by a linkage;
- and
- wherein, upon intracellular uptake of the nanoprobe, a linkage between the active agent and a respective quantum dot is cleaved to allow for release of the biologically active agent and to allow the plurality of quantum dots to transfer from a quenched state, wherein the luminescence of the plurality of quantum dots is quenched, to a luminescent state, and wherein the luminescence of the plurality of quantum dot is activated;
- the method comprising:
- confirming release of the biologically active agent by detecting a presence of the plurality of the quantum dot in the luminescent state.
4 more elements
An activatable nanoprobe comprising:
- a core component;
- an activatable active agent associated with the core component via a bond configured to be cleaved upon exposure to an endogenous compound.
Dependencies are inferred from the claim text.