Stress Resistant Plants
AssigneeBayer CropScience AG
InventorsMarc DE BLOCK, Michael Metzlaff, Véronique Gossele
Life of this patent
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Abstract
Stress tolerance in plants and plant cells is achieved by using nucleotide sequences encoding enzymes involved in the NAD salvage synthesis pathway and/or the NAD de novo synthesis pathway e.g. for overexpression in plants.
Claims29
A method for obtaining a plant with increased stress resistance comprising
- a. introducing a chimeric gene into a cells of a plant to obtain transgenic cells, said chimeric gene comprising the following operably linked DNA fragments:
- i. A plant-expressible promoter;
- ii. A DNA region coding for a plant-functional enzyme of the nicotinamide adenine dinucleotide salvage synthesis pathway selected from nicotinamidase, nicotinate phosphoribosyltransferase, nicotinic acid mononucleotide adenyl transferase or nicotinamide adenine dinucleotide synthetase;
- iii. A 3′end region involved in transcription termination and polyadenylation;
- b. regenerating said transgenic cells to obtain a population of transgenic plants;
- and
- c. selecting a plant from said population of transgenic plants which exhibits increased stress resistance or selecting a plant which exhibits a reduced level of reactive oxygen species or maintains a high level of NADH under stress conditions when compared to a similar non-transgenic plant.
Use of a DNA sequence encoding a plant functional enzyme of the nicotinamide adenine dinucleotide salvage synthesis pathway selected from nicotinamidase, nicotinate phosphoribosyltransferase, nicotinic acid mononucleotide adenyl transferase or nicotinamide adenine dinucleotide synthetase to increase the stress resistance of a plant.
Use of a DNA sequence encoding a plant functional enzyme of the nicotinamide adenine dinucleotide salvage synthesis pathway selected from nicotinamidase, nicotinate phosphoribosyltransferase, nicotinic acid mononucleotide adenyl transferase or nicotinamide adenine dinucleotide synthetase to decrease the level of reactive oxygen species in a plant or a plant cell under stress conditions or to maintain the level of NAD in a plant or plant cell under stress conditions.
Use of a DNA sequence encoding a protein comprising an amino acid sequence selected from the amino acid sequence of SEQ ID No.:2, SEQ ID No.:4, SEQ ID No.:6;
- SEQ ID No.:8, SEQ ID No.:10, SEQ ID No.:12;
- SEQ ID No.:14;
- SEQ ID No.:16, SEQ ID No.:18, SEQ ID No.:20, SEQ ID No.:
- 22, SEQ ID No.:24 or a protein having about 60% sequence identity and having the enzymatic activity of nicotinamide adenine dinucleotide salvage synthesis pathway to increase the stress resistance of a plant.
Use of a DNA sequence encoding a protein comprising an amino acid sequence selected from the amino acid sequence of SEQ ID No.:2, SEQ ID No.:4, SEQ ID No.:6;
- SEQ ID No.:8, SEQ ID No.:10, SEQ ID No.:12;
- SEQ ID No.:14;
- SEQ ID No.:16, SEQ ID No.:18, SEQ ID No.:20, SEQ ID No.:
- 22, SEQ ID No.:24 or a protein having about 60% sequence identity and having the enzymatic activity of nicotinamide adenine dinucleotide salvage synthesis pathway to decrease the level of reactive oxygen species in a plant or a plant cell under stress conditions or to maintain the level of NAD in a plant or plant cell under stress conditions.
Use of a DNA sequence comprising an nucleotide sequence selected from the nucleotide sequence of SEQ ID No.:1, SEQ ID No.:3, SEQ ID No.:5;
- SEQ ID No.:7, SEQ ID No.:9, SEQ ID No.:11;
- SEQ ID No.:13;
- SEQ ID No.:15, SEQ ID No.:17, SEQ ID No.:19, SEQ ID No.:21 or SEQ ID No.:23 to increase the stress resistance of a plant.
Use of a DNA sequence comprising an nucleotide sequence selected from the nucleotide sequence of SEQ ID No.:1, SEQ ID No.:3, SEQ ID No.:5;
- SEQ ID No.:7, SEQ ID No.:9, SEQ ID No.:11;
- SEQ ID No.:13;
- SEQ ID No.:15, SEQ ID No.:17, SEQ ID No.:19, SEQ ID No.:21 or SEQ ID No.:23 to increase the stress resistance of a plant. to decrease the level of reactive oxygen species in a plant or a plant cell under stress conditions or to maintain the level of NAD in a plant or plant cell under stress conditions.
Dependencies are inferred from the claim text.