# US 2014/0360403 A1 · Organic-Inorganic Composite Materials as a Result of Biomineralization > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2014/0360403 A1 - Document ID: US20140360403A1 - Status: Published application - Kind: A1 - Application number: 14/369,325 - Filed: 2012-12-19 - Published: 2014-12-11 - Earliest claimed: 2011-12-30 - Assignees: Leibniz-Institut fuer Neue Materialien gemeinnuetzige GmbH - Applicants: Leibniz-Institut fuer Neue Materialien gemeinnuetzige GmbH - Inventors: Ingrid Weiss; Eva Weber; Magdalena Eder; Eduard Arzt; Andreas Simon Schneider - Web page: https://patentgrep.com/patent/US20140360403A1 ## CPC - G01N 33/573: for enzymes or isoenzymes - C07K 14/435: from animals; from humans - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) ## Abstract A method for producing organic-inorganic composite materials includes at least one biomineralising protein and/or polypeptide-encoding recombinant polynucleotide being introduced into at least one host cell, preferably from the class of slime moulds, and the expressed protein and/or polypeptide influencing the formation of inorganic particles in an extracellular matrix of the host cell. ## Claims ### Claim 1 A method of identifying biomineralizing systems, comprising: a) introducing at least one recombinant polynucleotide into at least one host cell, said recombinant polynucleotide; a1) encoding at least one protein and/or polypeptide, and a2) being suitable for expressing the encoded protein and/or polypeptide in said host cell, b) expressing the protein encoded by the recombinant polynucleotide; and c) investigating a biomineralizing action of the expressed protein. ### Claim 2 (depends on 1) The method as claimed in claim 1, wherein the recombinant polynucleotide has at least one nucleotide sequence encoding an amino acid sequence for controlling localization and/or time of expression of the protein and/or polypeptide. ### Claim 3 (depends on 2) The method as claimed in claim 2, wherein the recombinant polynucleotide encodes a fusion protein comprising the protein and/or polypeptide and a signal sequence for controlling localization and/or time of expression of the protein and/or polypeptide. ### Claim 4 (depends on 1) The method as claimed in claim 1, wherein the first recombinant polynucleotide encodes a protein and/or polypeptide from table 2. ### Claim 5 (depends on 4) The method as claimed in claim 4, wherein the host cell is a cell from the class of slime molds (Eumycetozoa). ### Claim 6 (depends on 5) The method as claimed in claim 5, wherein the host cell is a cell of the genus Dictyostelium. ### Claim 7 (depends on 1) The method as claimed in claim 1, wherein investigating the biomineralizing action comprises contacting the host cell with a solution of at least one salt. ### Claim 8 (depends on 1) An organic-inorganic composite material obtained by a method of claim 1. ### Claim 9 A recombinant nucleic acid, comprising a recombinant nucleic acid that encodes a fusion protein comprising at least one protein and/or polypeptide with biomineralizing action and at least one amino acid sequence for influencing secretion of said fusion protein, and includes a corresponding promoter. ### Claim 10 (depends on 9) The recombinant nucleic acid as claimed in claim 9, wherein the encoded protein and/or polypeptide with biomineralizing action has a sequence which is at least 80% homologous to that of a protein and/or polypeptide from table 2. ### Claim 11 A recombinant protein and/or polypeptide, comprising a fusion protein of at least one protein and/or polypeptide with biomineralizing action and an amino acid sequence for influencing secretion of said fusion protein, and said protein and/or polypeptide with biomineralizing action has a sequence which is at least 80% homologous to that of a protein and/or polypeptide from table 2. ### Claim 12 (depends on 9) A host cell, comprising a recombinant nucleic acid as claimed in claim 9. ### Claim 13 (depends on 1) The method as claimed in claim 1, wherein the first recombinant polynucleotide encodes a protein and/or polypeptide from table 3. ### Claim 14 (depends on 5) The method as claimed in claim 5, wherein the host cell is a cell of the species Dictyostelium discoideum.