Baculovirus expression systems
AssigneeGenethon
InventorsLionel Galibert, Otto-Wilhelm Merten, Monique Van Oers, Christel Riviere
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Abstract
The present invention relates to an optimized baculovirus construct useful for the production of virus (-like) particles or viral vectors, in particular viral vectors for gene therapy.
Claims11
A recombinant baculovirus genome comprising the p26 and p74 baculoviral genes, wherein the baculoviral genes cathepsin, chitinase and p10 are disrupted.
A recombinant baculovirus genome, wherein the baculoviral genes cathepsin, chitinase, p26, p74 and p10 are disrupted, and wherein the recombinant baculovirus genome comprises a heterologous sequence coding a part of a viral vector or a virus-like particle.
A method for producing a viral vector or virus-like particle, comprising the step of culturing a adapted cell with one or several recombinant baculovirus each comprising a recombinant baculovirus genome harboring a heterologous sequence of interest useful for producing said viral vector or virus-like particle,
- wherein all recombinant baculoviruses comprise a recombinant baculovirus genome comprising the p26 and p74 baculoviral genes, wherein the baculoviral genes cathepsin, chitinase and p10 are disrupted.
A method for producing a viral vector or virus-like particle, comprising the step of culturing an adapted cell with one or several recombinant baculovirus each comprising a recombinant baculovirus genome harboring a heterologous sequence of interest useful for producing said viral vector or virus-like particle,
- wherein all recombinant baculoviruses comprise a recombinant baculovirus genome wherein the baculoviral genes cathepsin, chitinase, p26, p74 and p10 are disrupted.
Dependencies are inferred from the claim text.