Novel bacillus thuringiensis gene with lepidopteran activity
AssigneePioneer Hi-Bred International, Inc.
InventorsAndre Roger Abad, Hua Dong, Sue Lo, Xiaomei Shi, Thomas Chad Wolfe
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Abstract
The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
Claims23
An isolated nucleic acid molecule selected from the group consisting of:
- (a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:1;
- (b) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2;
- and
- (c) a nucleic acid molecule encoding a protein comprising an amino acid sequence characterized by at least 95% sequence identity to an amino acid sequence of (b);
- wherein the % identity is based on the entire sequence and is determined by GAP version 10 analysis under default parameters.
An isolated polypeptide with pesticidal activity, selected from the group consisting of:
- (a) a polypeptide comprising the amino acid sequence of SEQ ID NO:2;
- (b) a polypeptide that is encoded by the nucleotide sequence of SEQ ID NO:1;
- and
- (c) a polypeptide having at least 95% sequence identity to a polypeptide sequence of (a) or (b);
- wherein the % identity is based on the entire sequence and is determined by GAP version 10 analysis under default parameters.
A plant having stably incorporated into its genome a DNA construct comprising a nucleotide sequence that encodes a protein having pesticidal activity, wherein said nucleotide sequence is selected from the group consisting of:
- (a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:1;
- (b) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2;
- (c) a nucleic acid molecule encoding a protein comprising an amino acid sequence characterized by at least 95% sequence identity to an amino acid sequence of (b);
- wherein the % identity is based on the entire sequence and is determined by GAP version 10 analysis under default parameters;
- and
- wherein said nucleotide sequence is operably linked to a promoter that drives expression of a coding sequence in a plant cell.
A method for protecting a plant from a pest, comprising introducing into said plant or cell thereof at least one expression vector comprising a nucleotide sequence that encodes a pesticidal polypeptide, wherein said nucleotide sequence is selected from the group consisting of:
- (a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:1;
- (b) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2;
- and
- (c) a nucleic acid molecule encoding a protein comprising an amino acid sequence characterized by at least 95% sequence identity to an amino acid sequence of (b);
- wherein the % identity is based on the entire sequence and is determined by GAP version 10 analysis under default parameters.
Dependencies are inferred from the claim text.