Brassica Plant Comprising a Mutant Indehiscent Allele
AssigneeBayer CropScience AG
InventorsBenjamin LAGA, Bart Den Boer, Bart Lambert
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Abstract
This invention relates to crop plants of which the fruit dehiscence properties are modulated. More specifically the invention relates to improved methods and means for reducing seed shattering, or delaying seed shattering until after harvest, while maintaining at the same time an agronomically relevant treshability of the pods, and for increasing yield.
Claims80
A Brassica plant comprising at least two IND genes, or a cell, part, seed or progeny thereof, characterized in that it comprises at least two partial knockout mutant IND alleles in its genome.
A plant according to any one of the preceding claims, wherein the partial knockout mutant IND alleles are selected from the group consisting of ind-a1-EMS06, ind-a1-EMS09, ind-a1-EMS13, ind-c1-EMS04, ind-c1-EMS08 and ind-c1-EMS09.
A plant according to any one of the preceding claims, which further comprises at least one full knockout mutant IND allele in its genome.
A plant according to any one of the preceding claims, which is homozygous for the partial and/or for the full knockout mutant IND allele.
A plant according to any one of the preceding claims, which produces a significantly reduced amount of functional IND protein compared to the amount of functional IND protein produced by a corresponding plant not comprising mutant IND alleles.
A plant according to any one of the preceding claims, wherein the seed shattering of the plant is significantly reduced or delayed compared to the seed shattering of a corresponding plant not comprising mutant IND alleles.
A plant according to any one of the preceding claims, which is a plant from a Brassica crop species, preferably Brassica napus, Brassica juncea, Brassica carinata, Brassica rapa or Brassica oleracea.
A plant according to any one of the preceding claims, which is a plant from a Brassica oilseed species, preferably Brassica napus, Brassica juncea or Brassica rapa.
A plant, or a cell, part, seed or progeny thereof, comprising at least one partial knockout mutant allele of an IND gene in its genome, wherein the IND gene comprises a nucleic acid molecule selected from the group consisting of:
- (a) a nucleic acid molecule which comprises at least 90% sequence identity to SEQ ID NO:
- 1, SEQ ID NO:
- 3 from the nucleotide at position 46 to the nucleotide at position 633, SEQ ID NO:
- 3, SEQ ID NO:
- 5, or SEQ ID NO:
- 7;
- (b) a nucleic acid molecule encoding an amino acid sequence comprising at least 90% sequence identity to SEQ ID NO:
- 2, SEQ ID NO:
- 4 from the amino acid at position 16 to the amino acid at position 21 or SEQ ID NO:
- 4.
A partial knockout mutant allele of an IND gene, wherein the IND gene comprises a nucleic acid molecule selected from the group consisting of:
- (a) a nucleic acid molecule which comprises at least 90% sequence identity to SEQ ID NO:
- 1, SEQ ID NO:
- 3 from the nucleotide at position 46 to the nucleotide at position 633, SEQ ID NO:
- 3, SEQ ID NO:
- 5, or SEQ ID NO:
- 7;
- (b) a nucleic acid molecule encoding an amino acid sequence comprising at least 90% sequence identity to SEQ ID NO:
- 2, SEQ ID NO:
- 4 from the amino acid at position 16 to the amino acid at position 21 or SEQ ID NO:
- 4.
Brassica seed comprising a partial knockout ind allele selected from the group consisting of:
- seed comprising the ind-a1-EMS06 allele having been deposited at the NCIMB under accession number NCIMB 41570, seed comprising the ind-a1-EMS09 allele having been deposited at the NCIMB under accession number NCIMB 41571, seed comprising the ind-a1-EMS13 allele having been deposited at the NCIMB under accession number NCIMB 41572, seed comprising the ind-c1-EMS04 allele having been deposited at the NCIMB under accession number NCIMB 41575, seed comprising the ind-c1-EMS08 allele having been deposited at the NCIMB under accession number NCIMB 41573, seed comprising the ind-c1-EMS09 allele having been deposited at the NCIMB under accession number NCIMB 41574, and derivatives therefrom.
A seed comprising the ind-a1-EMS06, ind-a1-EMS09, ind-a1-EMS13, ind-c1-EMS04, ind-c1-EMS08 or ind-c1-EMS09 allele, reference seed comprising said allele having been deposited at the NCIMB under accession number NCIMB 41570, NCIMB 41572, NCIMB 41572, NCIMB 41574, NCIMB 41575 and NCIMB 41573, respectively.
A Brassica plant comprising at least two IND genes at two loci, or a cell, part, seed or progeny thereof, characterized in that it comprises two mutant IND alleles at one locus in its genome.
A method to increase the yield of Brassica plant comprising at least two IND genes, comprising introducing two mutant homozygous IND alleles in its genome.
Dependencies are inferred from the claim text.