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US 2014/0351966 A1Published application
Shortcut procedure of transgene integration by hypotonic shock into male germinal cells for gene expression and transgenesis
AssigneeNational Institute of Immunology
InventorsSubeer Suhash Majumdar, Abul Usmani, Nirmalya Ganguli
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Abstract
The present invention relates to a new method of gene integration in cells comprising the steps of: preparation of a suspension of poly nucleotide fragments comprising the desired gene in a hypotonic solution; administration of said suspension to cells or tissues; and maintenance of the cells or tissues in vitro or in its normal physiological condition. The invention also relates to a method of generating transgenic animal.
Claims14
A method of gene integration in cells comprising the steps of:
- a. preparation of a suspension of poly nucleotide fragments comprising the desired gene in a hypotonic solution;
- b. administration of the suspension of step (a) to cells or tissues;
- and
- c. maintenance of the cells or tissues in vitro or in its normal physiological condition.
1 more element
A method of generating a transgenic animal comprising the steps:
- a) preparation of a suspension of desired poly nucleotide fragments in a hypotonic solution;
- b) administration of the suspension of step (a) to germ cells or tissues;
- c) maintenance of the germ cells or tissues in vitro or in its normal physiological condition;
- and
- d) utilization of sperm generated from germ cells containing the desired polynucleotide fragments in natural mating or assisted reproductive technique (ART) to generate transgenic animals.
2 more elements
Dependencies are inferred from the claim text.