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AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsJames Chang, Colin Woon, Hung Pham
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One aspect of the present invention is a method of producing a decellularized composite tissue bioscaffold for musculoskeletal tissue interface reconstruction by physicochemically treating a musculoskeletal tissue interface isolated from allogeneic sources. In certain embodiments, such musculoskeletal tissue interfaces can also be isolated from xenogeneic sources. The method comprises treatment of the interface with detergents, chemical oxidants and ultrasonic energy, and wash steps in between to remove residual detergents as well as oxidants. The resulting bioscaffold may be freeze-dried or lyophilized, sterilized and aseptically packaged for subsequent use.
A method of producing a decellularized composite tissue bioscaffold,
A decellularized composite tissue bioscaffold for musculoskeletal tissue interface reconstruction in a mammal, said bioscaffold produced by
A method of treating a musculoskeletal tissue interface injury in a mammal, said method comprising grafting a decellularized composite tissue bioscaffold into said mammal, said bioscaffold having been produced by subjecting a composite tissue, that interfaces a soft tissue and a hard tissue, to treatments using oxidation, chemical lysis and ultrasonic energy for removing cells and cellular debris, wherein at least 90% of cells were removed from said interface tissue.
Dependencies are inferred from the claim text.