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US 2014/0350370 A1Published application
Glucose sensing assay
AssigneeThe Texas A&M University System
InventorsBrian M. Cummins, Gerard L. Coté
Life of this patent
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Abstract
The disclosure provides a ligand that competes with glucose for binding the protein Concanavalin A (ConA) and competitive binding assays incorporating the ligand. The competing ligand binds to the primary and part or all of the extended binding sites of Concanavalin A. These and other aspects of the disclosure are useful for glucose monitoring (e.g., continuous glucose monitoring (CGM)).
Claims31
A ligand for Concanavalin A, comprising:
- (a) a Concanavalin A binding component that binds to the primary glucose binding site and part or all of an extended binding site on Concanavalin A;
- and
- (b) a transduction component, wherein the binding component is coupled to the transduction component, and wherein the transduction component generates a detectable signal upon binding of the ligand to Concanavalin A.
A method for monitoring glucose in a sample, comprising:
- detecting the competitive binding of a ligand to Concanavalin A in the sample, wherein the ligand has an affinity toward the primary glucose binding site and at least a portion of an extended binding site of Concanavalin A, wherein the ligand competes with glucose for binding to the primary binding site of Concanavalin A, and wherein a detectable signal is provided by a transduction component upon binding of the ligand to Concanavalin A.
A glucose monitoring system, comprising:
- (a) Concanavalin A;
- (b) a ligand having an affinity toward the primary binding site and all or part of the extended binding site of Concanavalin A, wherein the ligand effectively competes with glucose for binding to Concanavalin A;
- and
- (c) a transduction component to signal the state of assay binding.
1 more element
Dependencies are inferred from the claim text.