Inorganic pyrophosphate and uses thereof
AssigneeThe Curators of the University of Missouri
InventorsPeter Sutovsky, Young-Joo Yi
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Abstract
The present invention provides a new and improved sperm stimulating additive comprising a certain amount of inorganic pyrophosphate (PPi). Addition of PPi in the media for human/animal in vitro fertilization (IVF) improves fertilization rate; addition of PPi in the semen extender for farm animal artificial insemination (AI) may improve pregnancy rates; furthermore, mamalian oocytes matured in vitro in a medium including PPi attain improved fertilization and developmental potential, while embryos cultured in medium supplemented with PPi have improved development to blastocyst.
Claims11
1-11. (canceled)
A semen sexing method, comprising:
- (a) separating a mixed sperm suspension in a first culture medium into a population of x-bearing or y-bearing sperm with the aid of an elutant medium;
- (b) preserving the x-bearing or y-bearing sperm in a second culture medium,
- wherein, inorganic pyrophosphate (PPi) is added to the first culture medium, the elutant medium or the second culture medium.
13-17. (canceled)
A method of in vitro fertilization (IVF) comprising contacting an oocyte with sperm in the precence of inorganic pyrophosphate (PPi).
A method of culturing an embryo comprising culturing an embryo in a media comprising inorganic pyrophosphate.
A method of artificial insemination comprising providing sperm and inorganic pyrophosphate (PPi) to the reproductive tract of a female.
A method of maturing an oocyte in vitro comprising culturing the oocyte in a media comprising inorganic pyrophosphate.
Dependencies are inferred from the claim text.