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US 2014/0350263 A1Published application
Rapid fluorescence tagging of glycans and other biomolecules with enhanced ms signals
AssigneeWaters Technologies Corporation
InventorsDarryl W. Brousmiche, Ying-Qing Yu, Matthew Lauber
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Abstract
Reagents comprising MS active, fluorescent molecules with an activated functionality for reaction with amines useful in tagging biomolecules such as N-glycans and uses thereof are taught and described.
Claims7
A compound of the structural Formula IV-A:
- or a salt or solvate thereof, wherein
- X═C or N;
- R1 is independently selected from O═C═N—,
- R2 is independently selected from —H, —C1-C8 alkyl, —C1-C8 cycloalkyl, halo, dialkylamino, CH2-dialkylamino, aminocarbonyl, alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—;
- and
- R3 and R4 are independently selected from —H, alkyl, alkyl amino, alkylsulfonic acid, alkyl phosphonic acid, wherein R3 or R4 is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid, and wherein R3 and R4 together with the nitrogen to which they are attached may form an optionally substituted 5- to 8-membered saturated or partially unsaturated ring but not when R1 is O═C═N—.
3 more elements
A compound of the structural Formula IV-B:
- or a salt or solvate thereof, wherein
- X═C or N;
- R1 is independently selected from O═C═N—,
- R2 is independently selected from —H, —C1-C8 alkyl, —C1-C8 cycloalkyl, halo, dialkylamino, CH2-dialkylamino, aminocarbonyl, alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—;
- and
- R3 and R4 are independently selected from —H, alkyl, alkyl amino, alkylsulfonic acid, alkyl phosphonic acid, wherein R3 or R4 is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid, and wherein R3 and R4 together with the nitrogen to which they are attached may form an optionally substituted 5- to 8-membered saturated or partially unsaturated ring but not when R1 is O═C═N—.
3 more elements
A compound of the structural Formula VIII-A:
- wherein
- X═C or N;
- R1 is independently selected from O═C═N—,
- R2 is independently selected from —H, —C1-C8 alkyl, —C1-C8 cycloalkyl, halo, dialkylamino, CH2-dialkylamino, aminocarbonyl, alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—;
- and
- R3 and R4 are independently selected from —H, alkyl, alkyl amino, alkylsulfonic acid, alkyl phosphonic acid, wherein R3 or R4 is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid, and wherein R3 and R4 together with the nitrogen to which they are attached may form an optionally substituted 5- to 8-membered saturated or partially unsaturated ring but not when R1 is O═C═N—.
3 more elements
A compound of the structural Formula VIII-B:
- wherein
- X═C or N;
- R1 is independently selected from O═C═N—,
- R2 is independently selected from —H, —C1-C8 alkyl, —C1-C8 cycloalkyl, halo, dialkylamino, CH2-dialkylamino, aminocarbonyl, alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—;
- and
- R3 and R4 are independently selected from —H, alkyl, alkyl amino, alkylsulfonic acid, alkyl phosphonic acid, wherein R3 or R4 is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid, and wherein R3 and R4 together with the nitrogen to which they are attached may form an optionally substituted 5- to 8-membered saturated or partially unsaturated ring but not when R1 is O═C═N—.
3 more elements
A compound of the formula:
Dependencies are inferred from the claim text.