Chromophoric polymer dots with narrow-band emission

A polymer dot comprising:

• a condensed chromophoric polymer comprising a narrow-band unit attached to the backbone or a sidechain of the polymer, wherein the narrow-band unit comprises an emission spectrum with a full width half maximum (FWHM) of less than about 70 nm, and wherein the backbone comprises a first general monomer, a second general monomer, or a combination thereof.

A method of making a polymer dot, the method comprising:

• providing a solvent solution comprising a chromophoric polymer, the chromophoric polymer being in an elongated coil form and comprising a narrow-band monomer, and wherein the chromophoric polymer comprises an emission spectrum with a full width half maximum (FWHM) of greater than about 70 nm;
• and
• mixing the solvent solution comprising the chromophoric polymer with a miscible solvent to form a condensed chromophoric polymer, wherein the condensed chromophoric polymer comprises an emission spectrum with a FWHM less than about 70 nm.

A method of making a polymer dot, the method comprising:

• providing a solvent solution comprising a chromophoric polymer, the chromophoric polymer being in an elongated coil form and comprising a narrow-band monomer, and wherein the chromophoric polymer comprises an emission spectrum with a full width half maximum (FWHM) of less than about 70 nm;
• and
• mixing the solvent solution comprising the chromophoric polymer with a miscible solvent to form a condensed chromophoric polymer, wherein the condensed chromophoric polymer comprises an emission spectrum with a FWHM less than about 70 nm.

A method of multiplex detection with a polymer dot, the method comprising:

• detecting the polymer dot with a detector system comprising a filter configured to pass a spectrum of light having a full width half maximum (FWHM) of less than about 70 nm, wherein the polymer dot comprises a condensed chromophoric polymer comprising a narrow-band monomer, the condensed chromophoric polymer having an emission spectrum that is substantially passed through the filter.

A method of making a polymer dot, the method comprising:

• providing a solvent solution comprising a chromophoric polymer, the chromophoric polymer being in an elongated coil form and comprising a narrow-band unit attached to the backbone or a sidechain of the polymer, wherein the narrow-band unit comprises an emission spectrum with a full width half maximum (FWHM) of greater than about 70 nm;
• and
• mixing the solvent solution comprising the chromophoric polymer with a miscible solvent to form a condensed chromophoric polymer, wherein the condensed chromophoric polymer comprises an emission spectrum with a FWHM less than about 70 nm.

A method of making a polymer dot, the method comprising:

• providing a solvent solution comprising a chromophoric polymer, the chromophoric polymer being in an elongated coil form and comprising a narrow-band unit attached to the backbone or a sidechain of the polymer, wherein the narrow-band unit comprises an emission spectrum with a full width half maximum (FWHM) of less than about 70 nm;
• and
• mixing the solvent solution comprising the chromophoric polymer with a miscible solvent to form a condensed chromophoric polymer, wherein the condensed chromophoric polymer comprises an emission spectrum with a FWHM less than about 70 nm.