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US 2014/0350066 A1Published application
Compositions and their uses for gene therapy of bone conditions
AssigneeUniversity of Massachusetts
InventorsEdward I. GINNS, Gary R. OSTROFF
Life of this patent
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Abstract
In certain preferred embodiments, the present invention provides compositions and methods for the treatment of bone conditions associated with low bone density. In preferred embodiments, the present invention provides compositions and methods for the treatment of osteoprotegerin-responsive conditions.
Claims32
A composition comprising:
- a payload molecule that comprises a nucleic acid selected from the group consisting of an oligonucleotide, an antisense construct, a siRNA, an enzymatic RNA, a mRNA, a recombinant DNA construct, a linear DNA fragment, a blocked linear DNA fragment and a mixture thereof;
- a payload trapping molecule selected from the group consisting of chitosan, polyethylenimine, poly-L-lysine, alginate, xanthan, hexadecyltrimethylammoniumbromide and mixtures thereof;
- and a carrier selected from a yeast glucan particle or a yeast glucan-mannan particle.
-16. (canceled)
A method of increasing osteoprotegerin expression in a cell, comprising the steps of:
- providing an effective amount of a delivery system comprising an extracted yeast cell wall defining an internal space and comprising about 6 to about 90 weight percent beta-glucan, a payload trapping molecule and a payload molecule, wherein the payload molecule is an expression vector comprising a control element operatively linked to an open reading frame encoding an osteoprotegerin or a functional equivalent thereof;
- contacting the cell with the delivery system;
- and
- expressing the osteoprotegerin.
1 more element
A method of making an osteoprotegerin delivery system comprising the step of:
- contacting a payload molecule that comprises a nucleic acid selected from the group consisting of an oligonucleotide, an antisense construct, a siRNA, an enzymatic RNA, a mRNA, a recombinant DNA construct, a linear DNA fragment, a blocked linear DNA fragment and a mixture thereof with a payload trapping molecule selected from the group consisting of chitosan, polyethylenimine, poly-L-lysine, alginate, xanthan, hexadecyltrimethylammoniumbromide and mixtures thereof;
- and a carrier selected from a yeast glucan particle or a yeast glucan-mannan particle.
Dependencies are inferred from the claim text.