Substrates, Peptide Arrays, and Methods
AssigneeVibrant Holdings, LLC
InventorsJohn J. Rajasekaran, Vasanth Jayaraman, Tianhao Wang, Kang Bei, Hari Krishnan Krishnamurthy
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Abstract
Disclosed herein are formulations, substrates, and arrays. Also disclosed herein are methods for manufacturing and using the formulations, substrates, and arrays. Also disclosed are methods for identifying peptide sequences useful for diagnosis and treatment of disorders, and methods for using the peptide sequences for diagnosis and treatment of disorders, e.g., celiac disorder. In certain embodiments, substrates and arrays comprise a porous layer for synthesis and attachment of polymers or biomolecules.
Claims30
An array of features attached to a surface at positionally-defined locations, said features each comprising:
- a collection of peptide chains of determinable sequence and intended length, wherein within an individual feature, the fraction of peptide chains within said collection having the intended length is characterized by an average coupling efficiency for the coupling steps of at least 92%, wherein the number of coupling steps is greater than two steps.
A method of producing an array of features, comprising:
- obtaining a substrate comprising a planar layer comprising a metal and having an upper surface and a lower surface;
- and a plurality of pillars operatively coupled to the layer in positionally-defined locations, wherein each pillar has a planar surface extended from the layer, wherein the distance between the surface of each pillar and the upper surface of the layer is between 1,000-5,000 angstroms, wherein the surface of each pillar is parallel to the upper surface of the layer, and wherein the plurality of pillars are present at a density of greater than 10,000/cm2;
- and
- coupling through a series of coupling reactions the features to the plurality of pillars, said features each comprising a collection of peptide chains of determinable sequence and intended length, wherein within an individual feature, the fraction of peptide chains within said collection having the intended length is characterized by an average coupling efficiency over all coupling steps of at least 92%, wherein the number of coupling steps is greater than two steps.
A substrate, comprising:
- a planar layer comprising a metal and having an upper surface and a lower surface;
- and
- a plurality of pillars operatively coupled to the layer in positionally-defined locations, wherein each pillar has a planar surface extended from the layer, wherein the distance between the surface of each pillar and the upper surface of the layer is between 1,000-5,000 angstroms, and wherein the plurality of pillars are present at a density of greater than 10,000/cm2.
A method of preparing a substrate for attachment of a coupling reagent, comprising:
- obtaining a substrate comprising a planar layer comprising a metal and having an upper surface and a lower surface;
- and a plurality of pillars operatively coupled to the layer in positionally-defined locations, wherein each pillar has a planar surface extended from the layer, wherein the distance between the surface of each pillar and the upper surface of the layer is between 1,000-5,000 angstroms, wherein the surface of each pillar is parallel to the upper surface of the layer, wherein a linker molecule is attached to the surface of each pillar, wherein the substrate is contacted with a photoactive formulation, and wherein the plurality of pillars are present at a density of greater than 10,000/cm2;
- and
- exposing the substrate to ultraviolet light.
Dependencies are inferred from the claim text.