Non-Emulsion Methods And Masked Biomolecules

A method for making an array, comprising:

• a) providing:
• i) a first element comprising a surface;
• ii) a second element comprising bottom and top surfaces, said second element further comprising a plurality of channels extending from said bottom to said top surfaces;
• iii) a third element comprising a surface, and
• iv) a solution comprising biomolecules, said biomolecules further comprising anchoring molecules attached thereto;
• b) contacting the surface of said first element with said solution under conditions such that at least a portion of said biomolecules attach to at least a portion of said surface via said anchoring molecule so as to create a modified surface comprising attached biomolecules;
• and
• c) positioning said second element between the surfaces of said first and third elements such that the bottom surface of said second element is on top of said modified surface, so as to create first regions defined by sealed channels, said first regions comprising unmasked attached biomolecules, and second regions comprising masked attached biomolecules, thereby making an array.