Enhancement of sensitivity and specificity of ketosteroids and keto or aldehyde containing analytes
AssigneeDH Technologies Development Pte. Ltd.
InventorsSubhakar Dey, Subhasish Purkayastha
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Abstract
A method, a labeling reagent, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids. The analytes can be medical or pharmaceutical compounds in biological matrices. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry.
Claims15
A method for mass analysis of an analyte from a biological matrix comprising:
- derivatizing an analyte comprising an aldehyde or ketone functional group, with a labeling reagent of formula (I):
- Y—(CH2)n—O—NH2 (I)
- where n is 2, 3, 4, 5, or 6 and Y is:
- each R4 is independently H or a C1-C18 alkyl which is branched or straight chain,
- m is an integer between 1 and 20, and
- X is an anion,
- or a salt or hydrate thereof, to form a labeled analyte;
- ionizing the labeled analyte at a low collision energy so as to produce one predominant signature ion fragment;
- and
- detecting the signature ion fragment by mass analysis.
A kit for analysis of ketosteroids comprising a set of mass labels comprising two or more compounds of formula (I):
- Y—(CH2)n—O—NH2 (I)
- where n is 2, 3, 4, 5, or 6 and Y is:
- each R4 is independently H or a C1-C18 alkyl which is branched or straight chain,
- m is an integer between 1 and 20, and
- X is an anion,
- or a salt or hydrate thereof,
- and one or more or a buffer, a reagent, a separation column, and instructions for carrying out an assay.
Dependencies are inferred from the claim text.