Diagnostic polymorphisms for cardiac disease
AssigneeMor Research Applications Ltd.
InventorsOffer AMIR, Basil S. LEWIS
Life of this patent
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Abstract
One or more polymorphisms, including single nucleotide polymorphisms (SNPs), or combinations thereof, for diagnosis of cardiac disease, such as heart failure and atrial fibrillation.
Claims31
A kit for diagnosis of cardiac disease in a subject, said kit comprising a plurality of primer polynucleotides labeled with a covalently bound detectable label for detecting a plurality of polymorphisms in a sample taken from the subject, wherein said primer polynucleotides are capable of selectively amplifying a sequence identified by one of SEQ ID NOs 1, 2, 4, 10, 12, 14, 20, 21, 22 and 44, such that each of said sequences identified by one of SEQ ID NOs 1, 2, 4, 10, 12, 14, 20, 21, 22 and 44 is selectively amplified by at least one pair of primer polynucleotides, wherein each primer polynucleotide has a length ranging from 15 nucleotides to 30 nucleotides, and wherein each primer polynucleotide is arranged such that a central site of said primer polynucleotide binds to a polymorphism at each of said sequences identified by one of SEQ ID NOs 1, 2, 4, 10, 12, 14, 20, 21, 22 and 44;
- wherein for SEQ ID NO:1, said polymorphism is an A at position 1166, for SEQ ID NO:
- 2, said polymorphism is a C at position 1166;
- for SEQ ID NO:4, said polymorphism is a G at position 221;
- for SEQ ID NO:
- 10, said polymorphism is an A at position 256;
- for SEQ ID NO:
- 12, said polymorphism is a C at position 256;
- for SEQ ID NO:
- 14, said polymorphism is a T at position 256;
- for SEQ ID NO:
- 20, said polymorphism is a T at position 301;
- for SEQ ID NO:
- 21, said polymorphism is a sequence AGAGGAGGA starting at position 266;
- for SEQ ID NO:
- 44, said polymorphism is a T at position 201;
- wherein detection of said plurality of polymorphisms through said selectively amplifying indicates said diagnosis of cardiac disease in the subject.
A kit for diagnosis of cardiac disease in a subject, said kit comprising a plurality of probe polynucleotides labeled with a covalently bound detectable label for detecting a plurality of polymorphisms in a sample taken from the subject, wherein each of said probe polynucleotides is capable of selectively hybridizing to a sequence identified by one of SEQ ID NOs 1, 2, 4, 10, 12, 14, 20, 21, 22 and 44, such that each of said sequences identified by one of SEQ ID NOs 1, 2, 4, 10, 12, 14, 20, 21, 22 and 44 is selectively hybridized by at least one probe polynucleotide, wherein each probe polynucleotide has a length ranging from 15 nucleotides to 30 nucleotides, and wherein each probe polynucleotide is arranged such that a central site of said probe polynucleotide binds to a polymorphism at each of said sequences identified by one of SEQ ID NOs 1, 2, 4, 10, 12, 14, 20, 21, 22 and 44;
- wherein for SEQ ID NO:1, said polymorphism is an A at position 1166, for SEQ ID NO:
- 2, said polymorphism is a C at position 1166;
- for SEQ ID NO:4, said polymorphism is a G at position 221;
- for SEQ ID NO:
- 10, said polymorphism is an A at position 256;
- for SEQ ID NO:
- 12, said polymorphism is a C at position 256;
- for SEQ ID NO:
- 14, said polymorphism is a T at position 256;
- for SEQ ID NO:
- 20, said polymorphism is a T at position 301;
- for SEQ ID NO:
- 21, said polymorphism is a sequence AGAGGAGGA starting at position 266;
- for SEQ ID NO:
- 44, said polymorphism is a T at position 201;
- wherein detection of said plurality of polymorphisms through said selectively hybridizing indicates said diagnosis of cardiac disease in the subject.
A kit for diagnosis of cardiac disease in a subject, said kit comprising a plurality of probe polynucleotides covalently bound to a substrate for detecting a plurality of polymorphisms in a sample taken from the subject, wherein each of said probe polynucleotides is capable of selectively hybridizing to a sequence identified by one of SEQ ID NOs 1, 2, 4, 10, 12, 14, 20, 21, 22 and 44, such that each of said sequences identified by one of SEQ ID NOs 1, 2, 4, 10, 12, 14, 20, 21, 22 and 44 is selectively hybridized by at least one probe polynucleotide, wherein each probe polynucleotide has a length ranging from 15 nucleotides to 30 nucleotides, and wherein each probe polynucleotide is arranged such that a central site of said probe polynucleotide binds to a polymorphism at each of said sequences identified by one of SEQ ID NOs 1, 2, 4, 10, 12, 14, 20, 21, 22 and 44;
- wherein for SEQ ID NO:1, said polymorphism is an A at position 1166, for SEQ ID NO:
- 2, said polymorphism is a C at position 1166;
- for SEQ ID NO:4, said polymorphism is a G at position 221;
- for SEQ ID NO:
- 10, said polymorphism is an A at position 256;
- for SEQ ID NO:
- 12, said polymorphism is a C at position 256;
- for SEQ ID NO:
- 14, said polymorphism is a T at position 256;
- for SEQ ID NO:
- 20, said polymorphism is a T at position 301;
- for SEQ ID NO:
- 21, said polymorphism is a sequence AGAGGAGGA starting at position 266;
- for SEQ ID NO:
- 44, said polymorphism is a T at position 201;
- wherein detection of said plurality of polymorphisms through said selectively hybridizing indicates said diagnosis of cardiac disease in the subject.
A method for diagnosis of cardiac disease in a subject, comprising contacting a plurality of primer polynucleotides labeled with a covalently bound detectable label for detecting a plurality of polymorphisms to a sample taken from the subject, wherein said primer polynucleotides are capable of selectively amplifying a sequence identified by one of SEQ ID NOs 1, 2, 4, 10, 12, 14, 20, 21, 22 and 44, such that each of said sequences identified by one of SEQ ID NOs 1, 2, 4, 10, 12, 14, 20, 21, 22 and 44 is selectively amplified by at least one pair of primer polynucleotides, wherein each primer polynucleotide has a length ranging from 15 nucleotides to 30 nucleotides, and wherein each primer polynucleotide is arranged such that a central site of said primer polynucleotide binds to a polymorphism at each of said sequences identified by one of SEQ ID NOs 1, 2, 4, 10, 12, 14, 20, 21, 22 and 44;
- wherein for SEQ ID NO:1, said polymorphism is an A at position 1166, for SEQ ID NO:
- 2, said polymorphism is a C at position 1166;
- for SEQ ID NO:4, said polymorphism is a G at position 221;
- for SEQ ID NO:
- 10, said polymorphism is an A at position 256;
- for SEQ ID NO:
- 12, said polymorphism is a C at position 256;
- for SEQ ID NO:
- 14, said polymorphism is a T at position 256;
- for SEQ ID NO:
- 20, said polymorphism is a T at position 301;
- for SEQ ID NO:
- 21, said polymorphism is a sequence AGAGGAGGA starting at position 266;
- for SEQ ID NO:
- 44, said polymorphism is a T at position 201;
- and detecting selective amplification with said primer polynucleotides to determine said diagnosis of cardiac disease in the subject.
A method for diagnosis of cardiac disease in a subject, comprising contacting a plurality of probe polynucleotides labeled with a covalently bound detectable label for detecting a plurality of polymorphisms to a sample taken from the subject, wherein each of said probe polynucleotides is capable of selectively hybridizing to a sequence identified by one of SEQ ID NOs 1, 2, 4, 10, 12, 14, 20, 21, 22 and 44, such that each of said sequences identified by one of SEQ ID NOs 1, 2, 4, 10, 12, 14, 20, 21, 22 and 44 is selectively hybridized by at least one probe polynucleotide, wherein each probe polynucleotide has a length ranging from 15 nucleotides to 30 nucleotides, and wherein each probe polynucleotide is arranged such that a central site of said probe polynucleotide binds to a polymorphism at each of said sequences identified by one of SEQ ID NOs 1, 2, 4, 10, 12, 14, 20, 21, 22 and 44;
- wherein for SEQ ID NO:1, said polymorphism is an A at position 1166, for SEQ ID NO:
- 2, said polymorphism is a C at position 1166;
- for SEQ ID NO:4, said polymorphism is a G at position 221;
- for SEQ ID NO:
- 10, said polymorphism is an A at position 256;
- for SEQ ID NO:
- 12, said polymorphism is a C at position 256;
- for SEQ ID NO:
- 14, said polymorphism is a T at position 256;
- for SEQ ID NO:
- 20, said polymorphism is a T at position 301;
- for SEQ ID NO:
- 21, said polymorphism is a sequence AGAGGAGGA starting at position 266;
- for SEQ ID NO:
- 44, said polymorphism is a T at position 201;
- and detecting selective hybridization with said probe polynucleotides to determine said diagnosis of cardiac disease in the subject.
A method for diagnosis of cardiac disease in a subject, comprising contacting a plurality of probe polynucleotides covalently bound to a substrate for detecting a plurality of polymorphisms to a sample taken from the subject, wherein each of said probe polynucleotides is capable of selectively hybridizing to a sequence identified by one of SEQ ID NOs 1, 2, 4, 10, 12, 14, 20, 21, 22 and 44, such that each of said sequences identified by one of SEQ ID NOs 1, 2, 4, 10, 12, 14, 20, 21, 22 and 44 is selectively hybridized by at least one probe polynucleotide, wherein each probe polynucleotide has a length ranging from 15 nucleotides to 30 nucleotides, and wherein each probe polynucleotide is arranged such that a central site of said probe polynucleotide binds to a polymorphism at each of said sequences identified by one of SEQ ID NOs 1, 2, 4, 10, 12, 14, 20, 21, 22 and 44;
- wherein for SEQ ID NO:1, said polymorphism is an A at position 1166, for SEQ ID NO:
- 2, said polymorphism is a C at position 1166;
- for SEQ ID NO:4, said polymorphism is a G at position 221;
- for SEQ ID NO:
- 10, said polymorphism is an A at position 256;
- for SEQ ID NO:
- 12, said polymorphism is a C at position 256;
- for SEQ ID NO:
- 14, said polymorphism is a T at position 256;
- for SEQ ID NO:
- 20, said polymorphism is a T at position 301;
- for SEQ ID NO:
- 21, said polymorphism is a sequence AGAGGAGGA starting at position 266;
- for SEQ ID NO:
- 44, said polymorphism is a T at position 201;
- and detecting selective hybridization with said probe polynucleotides to determine said diagnosis of cardiac disease in the subject.
Dependencies are inferred from the claim text.