Systems and Methods for Immunosorbent Assay for Single and Multiple Analytes

A method for immunologically analyzing a sample for a single analyte comprising the steps of:

• introducing scavenger antigens into a well in a first fixed position;
• introducing target antigens into a well in a second fixed position apart from the first fixed position of the scavenger antigens;
• introducing tagged antibodies into the well, the tagged antibodies in flow communication with both the first and second fixed positions;
• whereby flow communication of the tagged antibodies allows binding to occur first between the tagged antibodies and the target antigens in the second fixed position, and second, in the absence of sufficient target antigens, between the tagged antibodies and the scavenger antigens in the first fixed position;
• and
• detecting tagged antibodies bound to target antigens in the second fixed position in the well.

A method for immunologically analyzing a sample for multiple analytes comprising the steps of:

• introducing scavenger antigens into a well in a first fixed position;
• introducing target antigens into a well in a second fixed position apart from the first fixed position of the scavenger antigens;
• introducing bound pairs of scavenger antibodies and tagged primary antibodies into the well, the tagged antibodies in flow communication with both the first and second fixed positions;
• whereby flow communication of the tagged antibodies allows binding to occur first between the tagged primary antibodies and the target antigens in the second fixed position, and second, in the absence of sufficient target antigens, between the scavenger antibodies and the scavenger antigens in the first fixed position;
• and
• detecting tagged antibodies bound to target antigens in the second fixed position in the well.