Methods of detecting lung cancer
AssigneeCepheid
InventorsBernard Michot, Olivier Delfour
Life of this patent
Estimating the term…
Abstract
Methods of detecting lung cancer, such as non-small cell lung cancer, including squamous cell carcinoma and adenocarcinoma, are provided. Methods of detecting changes in the levels of one or more small RNAs associated with lung cancer are also provided. Compositions and kits are also provided.
Claims27
A method for detecting the presence of lung cancer in a subject, the method comprising detecting the level of at least one RNA selected from small U2, miR-720, miR-451, 13207, and 13750, in a sample from the subject, wherein detection of a level of small U2 that is greater than a normal level of small U2, or detection of a level of at least one RNA selected from 13207, miR-720, miR-451, and 13750 that is less than a normal level of the respective RNA, indicates the presence of lung cancer in the subject.
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A method of facilitating the diagnosis of lung cancer in a subject or the monitoring of therapy in a lung cancer patient, comprising detecting the level of at least one RNA selected from small U2, miR-720, miR-451, 13207, and 13750, in a sample from the subject, and communicating the results of the detection to a medical practitioner for the purpose of determining whether the subject has lung cancer or monitoring therapy in the lung cancer patient.
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A method of monitoring response to therapy in a lung cancer patient, comprising detecting the level of at least one RNA selected from small U2, miR-720, miR-451, 13207, and 13750, in a first sample from the subject taken at a first time point, and comparing the level of at least one RNA selected from small U2, miR-720, miR-451, 13207, and 13750 to the level of the respective RNA in a second sample from the patient taken at a second time point, wherein the second time point is prior to the first time point, and wherein a decrease in the level of small U2 or an increase in the level of at least one RNA selected from miR-720, miR-451, 13207, and 13750 in the first sample relative to the second sample, indicates that the lung cancer patient is responding to therapy.
A method for detecting the presence of lung cancer in a subject, comprising obtaining a sample from the subject, providing the sample to a laboratory for detection of the level of at least one RNA selected from small U2, miR-720, miR-451, 13207, and 13750, in the sample, receiving from the laboratory a communication indicating the level of the at least one RNA, wherein detection of a level of small U2 that is greater than a normal level of small U2, or detection of a level of at least one RNA selected from 13207, miR-720, miR-451, and 13750 that is less than a normal level of the respective RNA, indicates the presence of lung cancer in the subject.
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A composition comprising an oligonucleotide that comprises at least eight contiguous nucleotides that are complementary to (i) an RNA selected from small U2, miR-720, miR-451, 13207, and 13750, or (ii) a cDNA reverse-transcribed from an RNA selected from small U2, miR-720, miR-451, 13207, and 13750, wherein the oligonucleotide is between 8 and 30 nucleotides long.
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Dependencies are inferred from the claim text.