Identifying peptides at the single molecule level
AssigneeBoard of Regents, The University of Texas System
InventorsEdward Marcotte, Jagannath Swaminathan, Andrew Wllington, Eric Anslyn
Life of this patent
Estimating the term…
Abstract
The present invention relates to methods for identifying amino acids in peptides. In one embodiment, the present invention contemplates labeling the N-terminal amino acid with a first label and labeling an internal amino acid with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.
Claims43
A method of treating peptides, comprising:
- a) providing a plurality of peptides immobilized on a solid support, each peptide comprising an N-terminal amino acid and internal amino acids, said internal amino acids comprising lysine, each lysine labeled with a label, and said label producing a signal for each peptide;
- b) treating said plurality of immobilized peptides under conditions such that each N-terminal amino acid of each peptide is removed;
- and
- c) detecting the signal for each peptide at the single molecule level.
A method of treating peptides, comprising:
- d) providing a plurality of peptides immobilized on a solid support, each peptide comprising an N-terminal amino acid and internal amino acids, said internal amino acids comprising lysine, each lysine labeled with a first label, said first label producing a first signal for each peptide, and said N-terminal amino acid of each peptide labeled with a second label, said second label being different from said first label;
- e) treating said plurality of immobilized peptides under conditions such that each N-terminal amino acid of each peptide is removed;
- and
- f) detecting the first signal for each peptide at the single molecule level.
35-51. (canceled)
A method of identifying amino acids in peptides, comprising:
- a) providing a plurality of peptides immobilized on a solid support, each peptide comprising an N-terminal amino acid and internal amino acids, said internal amino acids comprising lysine, each lysine labeled with a first label, said first label producing a first signal for each peptide, and said N-terminal amino acid of each peptide labeled with a second label, said second label being different from said first label, wherein a subset of said plurality of peptides comprise an N-terminal lysine having both said first and second label;
- b) treating said plurality of immobilized peptides under conditions such that each N-terminal amino acid of each peptide is removed;
- and
- c) detecting the first signal for each peptide at the single molecule level under conditions such that said subset of peptides comprising an N-terminal lysine is identified.
A method of identifying amino acids in peptides, comprising:
- a) providing a plurality of peptides immobilized on a solid support, each peptide comprising an N-terminal amino acid and internal amino acids, said internal amino acids comprising lysine, each lysine labeled with a first label, said first label producing a first signal for each peptide, and said N-terminal amino acid of each peptide labeled with a second label, said second label being different from said first label, wherein a subset of said plurality of peptides comprise an N-terminal acid that is not lysine;
- b) treating said plurality of immobilized peptides under conditions such that each N-terminal amino acid of each peptide is removed;
- and
- c) detecting the first signal for each peptide at the single molecule level under conditions such that said subset of peptides comprising an N-terminal amino acid that is not lysine is identified.
Dependencies are inferred from the claim text.