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US 2014/0349858 A1Published application
Amplification of a sequence from a ribonucleic acid
AssigneeIbis Bioscience, Inc.
InventorsStanley T. Motley, Mark W. Eshoo
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Abstract
The present invention relates to methods and compositions for tagging, amplifying, purifying, and or characterizing of ribonucleic acid (RNA) in a sample. In particular, methods are provided for preparing RNA from a sample for subsequent analysis.
Claims18
A method of amplifying viral RNA of unknown sequence from a sample, comprising:
- (a) adding nucleic acid tag onto the 3′ end of the viral RNA;
- (b) hybridizing an oligonucleotide to the nucleic acid tag, wherein said oligonucelotide comprises a hybridization domain and a functional domain comprising an RNA polymerase promoter region;
- (c) synthesizing double stranded cDNA from the viral RNA and oligonucleotide;
- (d) amplifying the double stranded cDNA with an RNA polymerase to yield amplified antisense RNA;
- and
- (e) converting the amplified antisense RNA into amplified cDNA.
3 more elements
The method of claim , wherein synthesizing double stranded cDNA from the viral RNA and oligonucleotide comprises reverse transcribing a first strand of said cDNA.
Dependencies are inferred from the claim text.