T cell receptor display
AssigneesAdaptimmune Limited, Immunocore Limited
InventorsBent Karsten Jakobsen, Torben Bent Andersen, Peter Eamon Molloy, Yi Li
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Abstract
A proteinaceous particle, for example a bacteriophage, ribosome or cell, displaying on its surface a T-cell receptor (TCR). The displayed TCR is preferably a heterodimer having a non-native disulfide bond between constant domain residues. Such display particles may be used for the creation of diverse TCR libraries for the identification of high affinity TCRs. Several high affinities are disclosed.
Claims8
1-11. (canceled)
A phage particle displaying on its surface a dimeric T-cell receptor (dTCR) polypeptide pair, wherein the dTCR polypeptide pair comprises
- a first polypeptide wherein a TCR α or δ chain variable domain sequence is fused to N terminus of a TCR α chain constant domain extracellular sequence, and
- a second polypeptide wherein a TCR β or γ chain variable domain sequence is fused to N terminus of a TCR β chain constant domain extracellular sequence,
- wherein the first and second polypeptides are linked by a disulfide bond which corresponds to a native inter-chain disulfide bond present in native dimeric αβ TCRs.
A diverse library of phage particles as claimed in any of the preceding claims.
Dependencies are inferred from the claim text.