Metabolic control of seed germination
AssigneesOregon State University, Donald Danforth Plant Science Center
InventorsRoger Beachy, Hiroyuki Nonogaki
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Abstract
This invention provides Methods of conveniently controlling seed germination in genetically modified plants by the administration of a chemical modulator. The plant comprises a hormone-regulating gene placed under the control of a gene switch.
Claims18
1-102. (canceled)
A transgenic plant exhibiting controllable seed germination, comprising within its genome, and expressing, a heterologous polynucleotide encoding a 9-cis-epoxycarotenoid dioxygenase (NCED) and a polynucleotide encoding a trans-acting factor, wherein the polynucleotide encoding the 9-cis-epoxycarotenoid dioxygenase (NCED) is operably linked for expression to a promoter comprising a cis-element, wherein in the activity of the cis-element is regulated by the trans-acting factor, wherein the activity of the trans-acting factor with respect to the cis-element is regulated by a positive chemical modulator.
A genetically modified plant exhibiting controllable seed germination, comprising within its genome, and expressing, a heterologous polynucleotide encoding a 9-cis-epoxycarotenoid dioxygenase (NCED) selected from among a NCED6 and a NCED9 and a heterologous polynucleotide encoding an ecdysone receptor, wherein the polynucleotide encoding the 9-cis-epoxycarotenoid dioxygenase (NCED) is operably linked for expression to a promoter comprising an ecdysone response element that is regulated by the ecdysone receptor in the presence of a positive chemical modulator of the ecdysone receptor.
Dependencies are inferred from the claim text.