Rna-directed dna cleavage and gene editing by cas9 enzyme from neisseria meningitidis
AssigneesWisconsin Alumni Research Foundation, Northwestern University
InventorsErik J. Sontheimer, Yan Zhang, Alfonso Mondragon, Rakhi Rajan, James Thomson, Zhonggang Hou
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Abstract
Disclosed are components and methods for RNA-directed DNA cleavage and gene editing. The components include and the methods utilize a Cas9 protein from Neisseria and one or more RNA molecules in order to direct the Cas9 protein to bind to and optionally cleave or nick a target sequence.
Claims30
A method for modifying a target DNA sequence in a cell, the method comprising:
- (a) expressing a Cas9 protein from a Neisseria species or a variant protein thereof in the cell;
- and
- (b) expressing or transfecting an RNA in the cell wherein the RNA binds to the Cas9 protein or variant, and the RNA hybridizes to the target DNA sequence.
A recombinant Cas9 protein from a Neisseria species or a variant thereof comprising a nuclear localization signal.
A recombinant Cas9 protein from a Neisseria species or a variant thereof comprising a ligand or a tag for purifying or identifying the Cas9 protein.
Dependencies are inferred from the claim text.