In vivo delivery of nucleic acids to the liver or liver tissue
AssigneeVeritas Bio, LLC
InventorCatherine Pachuk
Life of this patent
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Abstract
The invention encompasses methods of delivering nucleic acids, including dsRNA, to mammalian target cells in vivo via intercellular transfer, wherein the dsRNA is delivered to or expressed in a first cell different from the target cell, wherein the first cell facilitates delivery of the dsRNA to the target cell.
Claims20
A nucleic acid or expression vector for use in delivering a nucleic acid or expression vector to a target organ or tissue in an animal comprising:
- transfecting at least one cell of a first tissue in said animal with the nucleic acid or expression vector, wherein said transfection results in said nucleic acid or expression vector being delivered to at least one cell in said target tissue or organ, wherein said target organ or tissue is distal from the site of said transfected first cell.
A nucleic acid or expression vector for use in delivering a nucleic acid or expression vector to a target cell in an animal comprising:
- transfecting a first cell in vitro with said nucleic acid or expression vector, isolating at least one exovesicle from said first cell wherein said exovesicle contains said nucleic acid or expression vector, and contacting said target cell with said exovesicle.
A nucleic acid or expression vector for use in delivering a nucleic acid or expression vector to a target organ or tissue in an animal wherein delivery comprises transfecting at least one first cell ex vivo with said nucleic acid or expression vector, and introducing said transfected cell into said animal, wherein said introduction results in production of an exovesicle containing said nucleic acid or expression vector and said nucleic acid or expression vector being delivered to at least one second cell in said target organ or tissue via said exovesicle, wherein said target organ or tissue is distal from the site of introduction of said transfected first cell.
A nucleic acid or expression vector for use in delivering a nucleic acid or expression vector to a cancer cell in an animal comprising:
- transfecting at least one cell of a first tissue in said animal with the nucleic acid or expression vector, wherein said transfection results in production of an exovesicle containing said nucleic acid or expression vector and said nucleic acid or expression vector being delivered to at least one cancer cell via said exovesicle, wherein said cancer cell is distal from the site of said transfected first cell.
A nucleic acid or expression vector for use in delivering a nucleic acid or expression vector to an HIV-infected cell in an animal comprising:
- transfecting at least one cell of a first tissue in said animal with the nucleic acid or expression vector, wherein said transfection results in said nucleic acid or expression vector being delivered to at least one HIV-infected cell, wherein said HIV-infected cell is distal from the site of said transfected first cell.
A nucleic acid or expression vector for use in delivering a nucleic acid or expression vector to a target organ or tissue in an animal for treating a patient suffering from an infection comprising:
- transfecting at least one cell of a first tissue in said animal with the nucleic acid or expression vector, wherein said transfection results in said nucleic acid or expression vector being delivered to at least one cell in said target tissue or organ, wherein said target organ or tissue is distal from the site of said transfected first cell.
Dependencies are inferred from the claim text.