System And Process For Separating A Material
AssigneeBiomet Biologics, LLC
InventorsRandel DORIAN, Michael D. Leach, Richard W. Storrs, Jason Chavarria
Life of this patent
Estimating the term…
Abstract
Disclosed is a system to separate, enrich, and/or purify a cellular population from a biological tissue, such as a tissue sample. For example, an adipose tissue sample can be acquired and disrupted. The disrupted tissue sample can then be separated and purified. The separated components can include multipotent, pluripotent, or other cell populations.
Claims22
A system to separate a selected cell fraction from a sample volume, comprising:
- a container having a first wall extending from a first bottom wall to a an upper rim having an interior surface and an exterior surface;
- a collection section positioned near the upper rim and at least contacting the exterior surface of the first wall, the collection section having a second wall extending from a second bottom wall to the upper rim, and wherein the second bottom wall is nearer the upper rim than the first bottom wall;
- a lid assembly extending over both the container and the collection section;
- a sealing section operable to seal a collection volume within the collection section from a container volume in the container;
- a flexible section operable to allow the sealing section to move between an open position and a closed position;
- and
- a drive motor operable to rotate the container and the collection section simultaneously;
- wherein in the closed position the sealing section selectively seals the collection volume from the container volume;
- wherein at a selected spin rate the sealing section moves to the open position from the closed position.
A system to separate a selected cell fraction from a sample volume, comprising:
- a container having a main container area and an isolation area spaced from the main container area with a wall portion therebetween;
- and
- a lid covering both the main container area and the isolation area, the lid including,
- a sealing portion operable to seal the main container area from the isolation area, and
- a flexible portion operable to allow the sealing portion to move between an open position and a closed position,
- wherein in the closed position the sealing position seals the main container area from the isolation area and in the open position the isolation area is opened to the main container area.
A system to separate a selected cell fraction from a sample volume, comprising:
- a container having a main container area and an isolation area spaced from the main container area with a wall portion therebetween, the isolation area forming an annular well around the main container area;
- a barrier wall extending a width of the isolation area, where the barrier wall defines a first end and a second end of the isolation area;
- and
- a lid covering both the main container area and the isolation area, the lid including,
- a sealing portion operable to seal the main container area from the isolation area, and
- a flexible portion operable to allow the sealing portion to move between an open position and a closed position,
- wherein in the closed position the sealing portion seals the main container area from the isolation area and in the open position the main container area is open to the isolation area.
Dependencies are inferred from the claim text.