Ultra-high-speed nucleic acid extracting apparatus and nucleic acid extracting method using same
AssigneeNanobiosys Inc.
InventorsSung Woo KIM, Duck Joong KIM, Dong Hoon LEE, Sun Jin KIM, Yong Hea CHOI, Ho Sun RYU
Life of this patent
Estimating the term…
Abstract
A nucleic acid extraction device, which can realize automation, ultra-miniaturization and super-high speed in the nucleic acid extraction reaction, has no limitation to the type of biological specimens that can be used, such as sputum, blood, cells, urine, saliva, tissues, etc., minimize the used amount of the sample solution, and also maintain and/or improve the nucleic acid extraction efficiency with reliability.
Claims19
A device for extracting a nucleic acid from a biological specimen, the device comprising:
- a substrate;
- a microfluidic chip comprising,
- an inlet portion,
- an outlet portion,
- a channel connecting the inlet portion and the outlet portion, and
- a filter disposed in the channel and having a porous structure;
- a chip support disposed on one side of the substrate and including a chip receiving portion defining a space so that the chip receiving portion houses the microfluidic chip therein;
- and
- a heater disposed in the substrate and configured to apply heat to a part of the microfluidic chip.
A device for extracting a nucleic acid from a biological specimen, the device comprising:
- a substrate;
- a microfluidic chip comprising,
- an inlet portion,
- a heating portion disposed in a first channel region connected to the inlet portion and configured to transfer the heat obtained from an exterior to the biological specimen introduced from the inlet portion;
- a first filter arranged in a second channel region connected to the heating portion and filtering a substance having a size larger than that of the nucleic acid out;
- a nucleic acid separating portion arranged in a third channel region connected to the first filter and having a nucleic acid binding substance capable of specifically binding with the nucleic acid;
- a second filter arranged in a fourth channel region connected to the nucleic acid separating portion and filtering the substance having a size larger than that of the nucleic acid out, and
- an outlet portion connected to the second filter;
- a chip support disposed on one side of the substrate and including a chip receiving portion defining a space so that the chip receiving portion houses the microfluidic chip therein;
- a heater disposed in the substrate and configured to apply heat to a part of the microfluidic chip;
- a chip cover being arranged on one side of the chip support and having a horizontal surface in close contact with another side of the microfluidic chip;
- a fluid control module configured to control the flow of a reactant solution in the microfluidic chip;
- and
- a heat control unit connected to the heater to control an amount of heat applied to a reactant solution in the microfluidic chip,
- wherein the chip receiving portion has a shape of a plate with a horizontal surface in close contact with one side of the microfluidic chip,
- wherein the heater is a contact plate type heating block, and
- wherein the heater transfers the heat to the microfluidic chip by a surface contact.
A device for extracting a nucleic acid from a biological specimen, the device comprising:
- a substrate;
- a microfluidic chip comprising a filter having a porous structure;
- and
- a chip support disposed on one side of the substrate and including a chip receiving portion defining a space so that the chip receiving portion houses the microfluidic chip therein;
- and
- a heater disposed in the substrate and configured to apply heat to a part of the microfluidic chip.
Dependencies are inferred from the claim text.