Lipase Variants and Polynucleotides Encoding Same
AssigneeNovozymes A/S
InventorsLuise Erlandsen, Carsten Hoerslev Hansen, Jesper Vind, Allan Svendsen, Carsten Peter Sonksen
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Abstract
The present invention relates to lipase variants and methods of obtaining them. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Claims25
A method for obtaining a lipase variant, comprising introducing into a parent lipase a substitution at one or more positions corresponding to positions T37, N39, or G91 of the mature polypeptide of SEQ ID NO:
- 2, wherein the variant has lipase activity and in comparison with the parent lipase has improved performance in the presence of an organic catalyst selected from the group consisting of organic catalysts having the following formulae:
- a)
- b)
- or
- c) mixtures thereof,
- wherein each R1 is independently a branched alkyl group containing from 3 to 24 carbons or a linear alkyl group containing from 1 to 24 carbons;
- and recovering the variant.
A lipase variant, comprising a substitution at one or more positions corresponding to positions T37A,D,E,F,G,H,I,L,N,P,Q,R,S,V,W,Y, N39A,C,D,E,F,G,I,K,L,M,P,Q,R,T,V,W,Y, and G91D,H,I,P,Q of the mature polypeptide of SEQ ID NO:
- 2, wherein the variant has lipase activity.
A lipase variant, wherein said variant is:
- a) G91A +D96G +T231R +N233R;
- b) G91A +D96G +G225R +T231R +N233R;
- c) G91N +E210Q +T231R +N233R +P250R;
- d) G91L +E210Q +T231R +N233R;
- or
- e) G91A +D96G +A150G +T231R +N233R;
- corresponding to the mature polypeptide of SEQ ID NO:
- 2, wherein the variant has lipase activity.
Dependencies are inferred from the claim text.