Novel vector containing multiple nucleotide sequences for the expression of enzymes
OwnerNorman Z. Lai, Fred Nyberg +1inventors
InventorsNorman Z. Lai, Fred Nyberg, Hung Mak
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Abstract
An expression vector is provided. The vector includes a promoter configured to drive the expression of the transgene in the cell. The vector also includes a tag sequence encoding a tag peptide directing the protein of the expressed transgene to a pre-determined location. The vector further includes a cleavage sequence encoding a peptide that is recognizable by a protease and a marker gene configured to encoding a protein to indicate the expression of the transgene.
Claims18
An expression vector for amplified expression of a transgene in a cell, comprising:
- a promoter configured to drive the expression of the transgene in the cell;
- a tag sequence encoding a tag peptide directing the protein of the expressed transgene to a pre-determined location;
- a first cleavage sequence encoding a peptide that is recognizable by a protease;
- and
- a marker gene configured to encoding a protein to indicate the expression of the transgene.
A process for preparation of proteins using a vector having a promoter configured to drive the expression of a transgene in the cell, a tag sequence encoding a tag peptide performing a function of facilitating the protein of the expressed transgene and locating the protein of the expressed transgene to a pre-determined location, a cleavage sequence encoding a peptide that is recognizable by a protease a multiple cloning site (MCS) including sequence recognizable by a restriction enzyme, and a marker gene configured to encoding a protein to indicate the expression of the transgene, comprising the steps of:
- inserting the transgene into the MCS site of the expression vector;
- introducing the vector having the transgene into a cell;
- culturing the cell;
- and
- expressing the transgene in the cell.
Dependencies are inferred from the claim text.