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US 2014/0349362 A1Published application
Microorganisms and processes for the production of isoprene
AssigneeGlycos Biotechnologies, Inc.
InventorsPaul Campbell, Sebastian Bredow, Huaijin Zhou, Stephanie Doneske, Daniel J. Monticello
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Abstract
The present invention provides a novel biosynthetic pathway for the production of isoprene from 3-methyl-2-buten-1-ol or 2-methyl-3-buten-2-ol. Further embodiments provide non-naturally occurring microorganism that have been modified to produce isoprene from 3-methyl-2-buten-1-ol or 2-methyl-3-buten-2-ol and methods of producing isoprene using said microorganism.
Claims12
A method of producing isoprene, the method comprising the steps of:
- a) optionally converting 3-methyl-2-buten-1-ol to 2-methyl-3-buten-2-ol using a 2-methyl-3-buten-2-ol isomerase enzyme;
- and
- b) converting 2-methyl-3-buten-2-ol to isoprene using a 2-methyl-3-buten-2-ol dehydratase enzyme.
Dependencies are inferred from the claim text.