Recombinant host cells comprising phosphoketolases
AssigneeButamax Advanced Biofuels LLC
InventorsMICHAEL DAUNER, Lori Ann Maggio-Hall, Jean-Francois Tomb
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Abstract
The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate, or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.
Claims30
1-4. (canceled)
A recombinant host cell comprising:
- (i) at least one genetic modification in an endogenous gene encoding a polypeptide having pyruvate decarboxylase activity, wherein the at least one genetic modification reduces or eliminates pyruvate decarboxylase activity;
- and
- (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity.
(canceled)
24-27. (canceled)
A method of improving the growth of a recombinant host cell comprising at least one genetic modification in an endogenous gene encoding a polypeptide having pyruvate decarboxylase activity, wherein the at least one genetic modification reduces or eliminates pyruvate decarboxylase activity, comprising:
- transforming said recombinant host cell with a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity,
- wherein the recombinant host cell comprising the heterologous polynucleotide encoding a polypeptide having phosphoketolase activity has improved growth as compared to a control recombinant host cell without the heterologous polynucleotide encoding a polypeptide having phosphoketolase activity.
A method of reducing the requirement for an exogenous two-carbon substrate for the growth of a recombinant host cell comprising at least one genetic modification in an endogenous gene encoding a polypeptide having pyruvate decarboxylase activity, wherein the at least genetic modification reduces or eliminates pyruvate decarboxylase activity, comprising:
- transforming said recombinant host cell with a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity, wherein the recombinant host cell comprising the heterologous polynucleotide encoding a polypeptide having phosphoketolase activity has a reduced requirement for an exogenous two-carbon substrate as compared to a control recombinant host without the heterologous polynucleotide encoding a polypeptide having phosphoketolase activity.
Dependencies are inferred from the claim text.